From 2012 to 2015 32 woodland grape genotypes were collected from the Szigetköz and the Fertő-Hanság National Park, Hungary, and ex-situ preserved in the gene bank of the NARIC Research Institute for Viticulture and Enology in Badacsony, Hungary. Phenological development stages based on the BBCH scale and ampelographic data according to 30 OIV descriptors were recorded. Isozyme and SSR analyses were carried out in the preserved genotypes, 18 Vitis vinifera varieties and 20 rootstocks were also analysed for comparison. Summarizing the results it can be estab-lished, that the ex-situ preserved genotypes are true-to-type woodland grapes, belonging to Vitis sylvestris Gmel. var. typica. Further quest, ex-situ and in-situ preservation and analyses of the Vitis sylvestris Gmel. germplasm in Hungary are needed.

The quest and conservation of existing populations of woodland grape (Vitis vinifera L. ssp. sylvestris Gmelin), the supposed progenitor of the European grapevine (Vitis vinifera L. ssp. sativa) and a significant actor in the evolution of grapevine, has great importance in preserving biodiversity. The proof of true-to-typeness is highly important in ex-situ conservation, because the contamination risk of the woodland grape populations is very high. Some characteristic "sylvestris" simple sequence repeats (SSR) alleles were identified, but they are only characteristic in a specific population. In our recent study, the SSR profiles of 32 woodland grapes were compared to those of 16 European grapevine varieties and 20 rootstocks. Morphology and SSR analyses suggested that the analysed Vitis vinifera ssp. sylvestris Gmelin accessions were true-to-type. In this report, the results of the acid phosphatase isoenzyme analyses of the same woodland grape accessions are presented and a new marker for true-to-typeness is suggested.