The analytical performance of the proposed laser-based photoacoustic spectroscopy (LBPAS) and optothermal window (OW) method for quantification of β-carotene content in sea buckthorn juices is compared to that of the conventional high-performance liquid chromatography (HPLC). Both photothermal techniques (laser-based photoacoustic spectroscopy (LBPAS) and optothermal window (OW)) are direct methods that unlike HPLC obviate the need for the extraction of the analyte. The outcome of the study leads to the conclusion that LBPAS and OW are both suitable for quick screening of ß-carotene in sea buckthorn juices. The β-carotene content of the samples changed due to the added sea buckthorn pomace and storage time. The correlation between the two methods and HPLC is linear with R2=0.9897 for LBPAS and R2=0.9928 for OW, respectively. Both methods are capable of the rapid and non-destructive determination of β-carotene in sea buckthorn juices without a need for sample pre-treatment.