European badgers are opportunistic animals that could serve as important hosts in the life cycle of hard ticks (Acari: Ixodidae) when entering peri-urban and urban environments. In this study, ticks and spleen samples were collected from badgers (Meles meles) found as roadkill between 2020 and 2021 in peri-urban habitats in Central Europe, Hungary. Altogether, 117 ticks, representing seven species (Ixodes ricinus, Ixodes kaiseri, Ixodes canisuga, Ixodes hexagonus, Haemaphysalis concinna, Alloceraea inermis and Dermacentor reticulatus) were removed from 49 badgers. Following assessment of suitability for obtaining spleen samples from the carcasses, DNA was extracted, and conventional or real-time PCRs were used to detect tick-borne pathogens in spleen samples of 38 badgers. Among protozoan parasites, two Babesia species, representing two phylogenetic groups, and Hepatozoon martis were identified. In addition, Candidatus Neoehrlichia lotoris, a novel Ehrlichia species (provisionally named as Candidatus Ehrlichia transdanubiensis), and Anaplasma phagocytophilum were detected in these tissue samples. The presence of these tick-borne pathogens in peri-urban mustelids indicate that they may provide a source for the infection of ixodid ticks which can in turn transmit these pathogens to humans or pet dogs in urban habitats. Thus, badgers pose an important epidemiological risk factor at the interface of sylvatic and synanthropic environments.