The operculated land snail family Pupinidae from mainland Southeast Asia has been systematically revised based on shell morphology. Despite previous morphological studies, the evolutionary relationships within this family remained unclear. This study represents the first comprehensive molecular phylogeny of this snail group, utilising two mitochondrial (COI and 16S rRNA) and two nuclear (5.8S rRNA + ITS2 and 28S rRNA) genetic markers. Additionally, we conducted phylogenetic analyses of Pupina species from 1106 loci generated through double-digest restriction site-associated DNA sequencing (ddRADseq). It turned out that Southeast Asian Pollicaria emerged as a sister clade to Central American Aperostoma of the Megalomastomatidae, leading to the resurrection of the Pollicariidae. Among the remaining pupinid genera, Tortulosa was nested within the Coptocheilus clade, while Pupina and Pupinella were not monophyletic. The previously recognised Pupina arula species group was found to be monophyletic and was reclassified into Tylotoechus (formerly a Pupina subgenus), based on distinctive conchological characters such as an extending parietal tooth from a parietal callus and a wide, outward-curving posterior canal. However, some Pupina and Tylotoechus species were not retrieved as monophyletic, suggesting the presence of multiple ‘cryptic species’. Divergence time estimation indicated that the Pupinidae split could date back to the Late Triassic to Early Cretaceous, with the first diversification of pupinid genera occurring during the Middle Jurassic and Early Cretaceous. This successful reconstruction of a robust phylogeny using ddRADseq loci demonstrates the significant potential of RADseq techniques in elucidating the evolutionary relationships of deeply divergent taxa. Further studies incorporating the type species Tylotoechus destructus and Pupina keraudrenii are necessary to justify the usage of these genera.

Hypselostomatidae is a large family of terrestrial pulmonate molluscs currently represented by 284 extant species, most confined to Southeast Asia. The current system of grouping species into genera is based on the morphology of the apertural barriers and the level of last whorl detachment. However, these characters overlap, challenging generic definitions. In this paper, these characters are evaluated and a novel classification proposed for hypselostomatid species belonging to the genera Anauchen (17 species), Bensonella (36 species), Boysidia (7 species, in part) and Hypselostoma (85 species). We assigned all species belonging to the genera Bensonella and Hypselostoma into two and four species groups respectively, which are characterised by combinations of morphological traits. Altogether 46 new species are described, seven species in Anauchen: A. crassus Gojšina, Hunyadi & Páll-Gergely, sp. nov., A. evanidus Gojšina & Páll-Gergely, sp. nov., A. grandiportus Gojšina, Grego & Páll-Gergely, sp. nov., A. obesus Gojšina, Hunyadi & Páll-Gergely, sp. nov., A. picasso Gojšina & Páll-Gergely, sp. nov., A. turritus Gojšina, Hunyadi & Páll-Gergely, sp. nov., A. jokaii Gojšina & Páll-Gergely, sp. nov.; 19 species in Bensonella: B. alycaeus Gojšina & Páll-Gergely, sp. nov., B. cardiostoma Gojšina, Vermeulen & Páll-Gergely, sp. nov., B. cristatissima Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. dha Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. dracula Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. exploda Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. fracta Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. microdentata Gojšina & Páll-Gergely, sp. nov., B. mitochondria Gojšina, Vermeulen & Páll-Gergely, sp. nov., B. mirabilis Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. montawa Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. multidentata Gojšina, A. Reischütz & Páll-Gergely, sp. nov., B. nitens Gojšina & Páll-Gergely, sp. nov., B. obex Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. perfecta Gojšina & Páll-Gergely, sp. nov., B. sericata Gojšina & Páll-Gergely, sp. nov., B. serrata Gojšina, Hunyadi & Páll-Gergely, sp. nov., B. spelaea Gojšina, Grego & Páll-Gergely, sp. nov., B. spinosa Gojšina, Hunyadi & Páll-Gergely, sp. nov.; 20 species in Hypselostoma: H. aquila Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. bubalus Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. circumcarinatum Gojšina, Auffenberg & Páll-Gergely, sp. nov., H. coriaceum Gojšina & Páll-Gergely, sp. nov., H. aenigma Gojšina, Grego & Páll-Gergely, sp. nov., H. fortunatum Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. fungus Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. geckophilum Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. iunior Gojšina & Páll-Gergely, sp. nov., H. ophis Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. platybasis Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. populare Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. sculpturatum Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. similare Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. sorormajor Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. sororminor Gojšina, Hunyadi & Páll-Gergely, sp. nov., H. torta Gojšina, Auffenberg & Páll-Gergely, sp. nov., H. vesovici Gojšina & Páll-Gergely, sp. nov., H. vicinum Gojšina, Auffenberg & Páll-Gergely, sp. nov., H. vujici Gojšina & Páll-Gergely, sp. nov. One replacement name is proposed: H. tertiusfrater Gojšina & Páll-Gergely, nom. nov. pro Boysidia salpinx F. G. Thompson & Dance, 1983, non Hypselostoma salpinx (van Benthem Jutting, 1961) (originally described as Gyliotrachela). Gyliotrachela and Antroapiculus are both treated as junior synonyms of Hypselostoma. An additional 28 species and subspecies are reassigned to the synonymies of other taxa.