3D printing technologies are now extensively utilized across numerous industries and scientific fields due to their ability to facilitate the practical testing of various design concepts cost-effectively and sustainably. This research focuses on PLA-based materials, known for their ease of recyclability. FDM printing technology was employed to identify the optimal printing layout for 3D-printed reinforcement elements in concrete, as this is not a standard parameter. Various material compositions and layout structures were analyzed using multiple printing strategies to find the most effective combination for innovatively enhancing the stiffness and strength of concrete structures. The GOM Aramis 5M DIC system, coupled with FDM printing technology, was used in this investigation, marking a novel approach in civil engineering.