Current Blood Glucose (BG) monitoring techniques are invasive or semi-invasive and can impose financial and practical burden on patients. In this study a compact, non-invasive and single port Substrate Integrated Waveguide (SIW) loaded with X-shape has been present. The sensor resonant at 1.7 GHz, a dual parameter employed to evaluate the sensor by tracking the shift of resonance frequency in (MHz) and the reflection coefficient (dB) to detect glucose-induced changes in tissue permittivity. The device is designed using full-wave electromagnetic simulations with multilayer tissue models and validated experimentally on five human volunteers under controlled fasting and post-glucose conditions. Across the physiological range of 20–200 mg/dL, the sensor exhibits sensitivities up to 0.310 MHz per mg/dL and 0.333 dB per mg/dL, demonstrating consistent responsiveness to glucose variations. The results indicate that the proposed resonator can track glucose-related dielectric changes using a simple contact-based configuration. However, the measurements are influenced by subject-specific variability and sensor placement conditions, which currently limit generalization and repeatability. Further work is required to improve robustness, calibration, and validation on larger cohorts before practical deployment.