Investigation of viral diseases of garlic (Allium sativum L.), new primers for RT-PCR detection and diversity of garlic viruses in Hungary

Publication Name: Physiological and Molecular Plant Pathology

Publication Date: 2024-11-01

Volume: 134

Issue: Unknown

Page Range: Unknown

Description:

The study investigated the presence of potyviruses, carlaviruses, and allexiviruses in garlic samples, highlighting the impact of these viruses on garlic cultivation. No virus-free garlic was identified, with infections from at least one virus species observed in all samples. Notably, LYSV was detected in one sample, emphasizing the critical need for certified propagating material. Our study revealed the predominance of allexiviruses, particularly GarVD. Interestingly, no mixed carlavirus infections were detected. The results underline the risk of virus complexes, particularly when potyvirus infections occur alongside carla- or allexiviruses, exacerbating yield losses. The new primers we designed were successfully used, the usability of certain primers from earlier research has been confirmed, and new potential applications have been identified for some primers. In our study we have molecularly identified LYSV, GCLV, SLV, GarVB, GarVC and GarVD infected samples from Hungary, the presence of LYSV was confirmed with ELISA as well.

Open Access: Yes

DOI: 10.1016/j.pmpp.2024.102394

Authors - 5