A new oribatid mite subgenus of the genus Amboroppia (Oribatida, Oppiidae) –A. (Quintanoppia) subgen. n., with A. (Quintanoppia) defectofossulata sp. n. as type species – is described, based on material collected from Mexico. It can be distinguished from the nominate subgenus by the absence concavities between rostrum and transcostula and behind transcostula, and the presence of strong teeth on lateral sides of prodorsum. A revised generic diagnosis, identification key and data on distribution and habitats of representatives of Amboroppia are presented.