This study aims to analyze various methods, including AI, that can be used to optimize error analysis in digital twins and highlight the advantages and disadvantages of these analysis methods. Furthermore, the study aims to present an automated solution for error analysis of MATLAB/Simulink-based digital twins. This solution can make the error analysis more efficient without the use of AI, meaning that it can be used even if the digital twin is not appropriately known, which can be a considerable advantage in the current automotive industry, where complex digital twins are commonly used for the development and optimization of E/E systems during different types of in-the-Loop simulations.