K. Szenthe

23010570300

Publications - 3

A rapid and efficient DNA isolation method for qPCR-based detection of pathogenic and spoilage bacteria in milk

Balázs Ásványi K. Szenthe István Gyurján Barbara Bánkuti Bettina Pancza Zsófia Sipos-Kozma Miklós Szathmáry Zoltán Tudós F. Bánáti Susan Szathmary László Stipkovits László Varga

Publication Name: Food Control

Publication Date: 2021-12-01

Volume: 130

Issue: Unknown

Page Range: Unknown

Description:

The objective of this study was to find an efficient, rapid, simple, and cost-effective method of pretreating raw milk samples to produce PCR-ready DNA for subsequent microbial detection using the strains of eight bacterial species. A total of 17 in-house protocols and three commercial kits were evaluated in three steps from scientific, practical, and economic perspectives. The results showed that an in-house procedure involving Triton X-100-based pretreatment and an inhibitor removal resin was superior to all other methods tested in terms of DNA yield, sensitivity, ease of sample handling, time efficiency, and cost per sample. Overall, this simplified preanalytical protocol was shown to have a great potential for use in rapid detection of dairy-related bacterial species, thereby enabling early intervention in the food chain and thus reducing the risk of negative economic and health outcomes.

Open Access: Yes

DOI: 10.1016/j.foodcont.2021.108236

Phylogenetic re-evaluation of previously identified Chlamydomonas (Chlorophyta, Chlamydomonadaceae) strains from The Mosonmagyaróvár Algal Culture Collection, Hungary, using molecular data

K. Szenthe H. D. Laughinghouse Péter Bálint B. Pap Gergely Maróti Zoltán Molnár D. E. Berthold S. Katona C. Kilgore N. Horváth Vince Ördög F. Bánáti L. Vörös

Publication Name: South African Journal of Botany

Publication Date: 2019-09-01

Volume: 125

Issue: Unknown

Page Range: 16-23

Description:

Systematic studies on 70 MACC isolates previously identified as ‘Chlamydomonas’, a unicellular flagellate, were carried out based on partial 18S rRNA. The aim of this study was to determine the phylogenetic affiliations of Chlamydomonas strains in the MACC collection. The study found that most of the strains were not Chlamydomonas. Nine clusters of phylogenetically similar taxa were identified. The previous determinations were completed with their new phylogenetic affiliations (partly due to changes in green algae classification). Molecular data revealed that 3 of the 70 strains are from Arenicolinia, 14 are members of the phylogroup Stephanosphaerinia, 11 are Oogamochlamydinia, 1 is Chloromonadinia, 19 are Reinhardtinia, 2 are Polytominia, 9 are Scenedesmaceae, 5 are Moewusinia, and 6 are Chlorella. Clades were established by 18S rRNA similarity and p-distances. This study reveals the need to revise established culture collections whose isolates are solely identified with morphology.

Open Access: Yes

DOI: 10.1016/j.sajb.2019.06.028

The reclassification of 37 strains from The Mosonmagyaróvár Algal Culture Collection, Hungary, which were previously identified as Anabaena (Cyanobacteria, Nostocaceae)

K. Szenthe H. D. Laughinghouse Péter Bálint B. Pap Gergely Maróti Zoltán Molnár D. E. Berthold S. Katona C. Kilgore N. Horváth Vince Ördög F. Bánáti L. Vörös

Publication Name: South African Journal of Botany

Publication Date: 2019-07-01

Volume: 123

Issue: Unknown

Page Range: 333-340

Description:

Study on 37 MACC isolates previously identified as “Anabaena,” a freshwater filamentous heterocytous taxon, were carried out using the 16S rRNA. The study found that most of the strains were misidentified at genus level. Three clusters of phylogenetically and morphologically similar taxa were identified. The previous determinations were amended with their new taxonomic classifications (partly due to changes in cyanobacterial classification). Some morphological structures could not be found in the cultures (e.g. akinetes). Molecular data revealed that 6 of the 37 strains are Desmonostoc, 8 are members of the genus Nostoc, 19 strains bear genetic resemblance to the genus Trichormus and 4 strains remain unresolved. Clades were established by 16S rRNA similarity and p-distances. The goal of this study was to amend the strain designations in this collection. This study reveals the necessity to revisit established culture collections that originally used only morphological classifications for species identification.

Open Access: Yes

DOI: 10.1016/j.sajb.2019.03.014