Air guns generate a large number of shot injuries, while pellets can produce life-threatening or lethal injuries by penetrating deep tissue. The assessment of air gun injuries include determining the direction and the distance of the shot, the type of arm, and the penetration capability of the pellet. Answering these questions can be challenging since air gun injuries have received less attention in scientific studies. This study aimed to determine skin and soft-tissue penetration capabilities and wound characteristics of air guns at different impact energies. Test shots were conducted on porcine tissue and ballistic gel with 0.177 caliber air guns from different shooting distances between impact energies of 3.28 and 15.71 J (111 m/s to 243.5 m/s). Entrance wound appearance, size, and penetration depth were compared. The penetration capabilities of the pellet with different impact energies are assessed and discussed. No difference was found in the entrance wound shape and appearance between shots from different distances and impact energy. The results indicate that 0.177 caliber air guns above 7.5 J of muzzle energy can easily penetrate deep into soft tissue, thus able to cause severe, life-threatening injuries. An air gun with a muzzle energy below 7.5 J muzzle energy can still cause severe injury up to 20–30 m as the projectile has enough energy to create several centimeter-deep penetrations.

Formalin-fixed paraffin-embedded (FFPE) tissue blocks are commonly used in the field of pathology and forensic pathology as a source of histological slides. For postmortem kinship analysis or identification, DNA can be extracted from blocks with specialized kits. However, when an STR profile should be generated from single microscope slides, the removal of the coverslip and the limited sample size poses unique challenges. We aimed to test the effectivity of agitated xylene incubation to dissolve the mounting material to facilitate the coverslip removal. DNA extraction tests were performed on 5- to 7-year-old histological slides. Xylol was used to dissolve the mounting medium to facilitate cover slide removal, one set of samples was shaken during incubation, and the other set was left still. It was found that shaking the sample while bathed in xylol decreased the incubation time from three days to two days. Agitation not just reduced the processing time but increased the quality of acquired STR profiles: on average 30% more alleles were detected from the shaken samples compared to the still bathed ones.

The anthropocentric nature of forensic sciences has been changing continuously over the years and this process is continuing today. Due to its universality and multilateral implementation, and the fragmented nature of forensic epistemology, the information provided by forensic genetics can play a pivotal role in forensic science. At the same time, the link between forensic genetics and non-human forensic biological evidence has become unquestionable. It may highlight the modern requirements of forensic science, and this connection is also able to provide useful and sufficient examples for developmental processes in wildlife forensics. Obviously, the local formations, organizations, and operations of wildlife forensics can be different worldwide, but the detection and punishment of wildlife-related criminal behavior, as well as the prevention of further crimes, play a relevant role in these processes everywhere.

The traditional air gun pellet is either spherical or diabolo type with a head followed by a narrow mid-section and a skirt. But apart from these traditional homogenous pellets, special ammunition is also available for air guns, including lead-free, dart-shaped, plastic-coated, and “explosive” pellets producing flash and loud bang upon impact. The scientific data on the penetration capability and terminal ballistic behavior of these pellets are very limited. The study aimed to determine and compare the ballistic gel and tissue penetration capabilities of different air gun ammunition. Ballistic gel and porcine skin penetration of eleven types of 4.5 mm diameter (.177) air gun pellets, including traditional diablo-type lead pellets, lead-free pellets, plastic-coated pellets, and ammunition with an “explosive” charge, were examined. The ballistic gel penetration depth was measured using a digital caliper. The porcine test targets underwent computer tomography (CT) examination and the traditional autopsy method. Histological analysis was performed of the entrance wound and wound track of “explosive” ammunition. The muzzle velocity ranged from 75.4 to 245.1 m/s, and the muzzle energy ranged from 2.5 to 8.5 J. The maximal gel penetration ranged from 26.0 to 117.8 mm, and the final projectile position ranged from 23.8 to 113.2 mm. Tissue penetration ranged from 8.5 mm to 102.2 mm, and full-thickness tissue penetration was achieved in 35 shots from 79. Results: indicate large variations in muzzle energy in the case of non-traditional pellets, even if the same weapon is used. Penetration depth does not correlate strongly with muzzle speed in the case of non-traditional pellets, as the shape and type of pellet have a significant influence on penetration. Some non-traditional pellets may leave behind their plastic sheath in the tissues, which requires special attention since these are invisible on CT scans. Pellets with an “explosive charge” leave behind a blackish discoloration around the entrance wound and in the wound track, which is visually identifiable and also visualized in CT.