This study investigates the enzymatic hydrolysis of soybean (Glycine max L.) and mung bean (Vigna radiata) proteins using bromelain, ficin, papain, and pepsin to improve digestibility and functional properties. We hypothesized that mung bean's less compact structure would yield higher degree of hydrolysis (DH) and bioactive peptides compared to soybean, enhancing antioxidant capacity for functional foods. Mung bean showed significantly higher proteolysis, with a maximum DH of 46.5 ± 2.1 % (p ≤ 0.05) using 10 % bromelain for 12 h, versus soybean's 26.9 ± 1.5 % (p ≤ 0.05). Bromelain and ficin outperformed papain and pepsin, producing up to 62.3 ± 3.2 % oligopeptides and 32.4 g/100 g free amino acids in mung bean. Mung bean hydrolysates exhibited superior antioxidant activity, reaching 78.4 ± 2.5 % DPPH scavenging (p ≤ 0.05), compared to soybean's 58.9 ± 2.0 % (p ≤ 0.05), due to increased 200–1000 Da peptides. Optimal conditions (10 % enzyme, 12 h) improved solubility and bioactivity, highlighting mung bean's potential and bromelain's efficacy for sustainable food applications, warranting further protease research.

Antioxidants play a crucial role in mitigating oxidative stress and preventing cellular damage caused by free radicals. This study aimed to compare the effectiveness of three antioxidant assays—DPPH, TEAC, and FRAP—in quantifying the antioxidant capacity of 15 plant-based spices, herbs, and food materials from five distinct plant families. The relationship between these assays and total polyphenol content (TPC) as well as total flavonoid content (TFC) was also investigated. The results showed that FRAP exhibited the strongest correlation with TPC (r = 0.913), followed by TEAC (r = 0.856) and DPPH (r = 0.772). Lamiaceae species, such as rosemary and thyme, consistently demonstrated high antioxidant activities across all assays. The study highlights the complementary nature of these assays in assessing antioxidant capacity and underscores their utility in profiling polyphenol- and flavonoid-rich plants for potential nutritional and therapeutic applications.

This study investigated the effects of elevated copper levels on the early-stage growth and development of three Arundo donax (giant reed) ecotypes (STM, BL, and ESP) from different climatic zones, focusing on plant morpho-physiological and copper biochemical changes (including root structure, photosynthetic structure, copper accumulation and translocation, soluble protein, and lipid peroxidation). Plants were grown under increasing concentrations of copper (0, 100, 200, 300, and 400 mg/kg), revealing that copper accumulation was predominantly localized in the roots, with ESP showing the highest at 1829 μg/g, followed by STM (1191 μg/g) and BL (935 μg/g) at 400 mg/kg. While morphological traits like plant height and stem diameter were less affected, root volume decreased significantly at high copper levels (e.g., by 60 % in BL from 10.00 cm³ in control to 4.00 cm³ at 400 mg/kg). Physiological responses varied significantly: photosynthetic pigments increased with moderate copper levels (e.g., chlorophyll a in BL from 31.67 μg/cm² in control to 49.19 μg/cm² at 400 mg/kg) but declined at higher concentrations in ESP. Lipid peroxidation, measured by malondialdehyde (MDA), indicated increased oxidative stress, especially in STM and ESP (e.g., root MDA in STM from 14.22 nmol/g in control to 26.30 nmol/g at 400 mg/kg). These results highlight the ESP ecotype's higher tolerance and copper sequestration capabilities, making it a promising candidate for further studies in copper-stressed environments.