Judit Süle

56015792000

Publications - 5

Aflatoxin M1 detection in raw milk and drinking milk in Hungary by ELISA − A one-year survey

Attila Hucker Róbert Kocsis G. Szafner Solveig Bukovics Judit Süle A. J. Kovács Katalin Szabó Henrietta Buzás Sárvári Loretta Csilla Katalin Nagy-Kovács

Publication Name: Journal of Food Composition and Analysis

Publication Date: 2023-08-01

Volume: 121

Issue: Unknown

Page Range: Unknown

Description:

The aim of this study was to monitor the aflatoxin M1(AFM1) contamination in raw milk and drinking milk in Hungary over a one-year period. A total of 474 milk samples of raw milk (n = 278) and commercial milk (n = 196) were collected and analysed between September 2021 and November 2022. Enzyme-Linked Immunosorbent Assay (ELISA) determined the concentration of AFM1. It was found that 68.7% (191/278) of the raw cow milk samples were contaminated by AFM1 in the range 5.0–173 ng/L, the mean of the positive samples was 30.7 ± 24.7 ng/L, and the median was 21.8 ng/L. The percentage of contamination in drinking milk was 79.1% (155/196). The mean, median, and range of the positive samples were 18.0 ± 10.9 ng/L, 16.18 ng/L, and 5.3–100 ng/L, respectively. Overall, 9.4% (26/278) of raw milk samples and only 1 commercial milk sample of 196 (0.5%) contained AFM1 exceeding the maximum residue level (MRL) of 50 ng/L set by the European Union. Our study suggests that based on calculated AFM1-related health risk indicators, the Hungarian adult population are not exposed to high levels of AFM1, but regular monitoring of aflatoxins is necessary not only for dairy farmers but also for the milk processing sectors.

Open Access: Yes

DOI: 10.1016/j.jfca.2023.105368

Tejsavbaktérium-izolátumok egyes probiotikus tulajdonságainak in vitro vizsgálata

Judit Süle Zoltán Hatvan Z. Kerényi László Varga

Publication Name: Elelmiszervizsgalati Kozlemenyek

Publication Date: 2023-01-01

Volume: 69

Issue: 1

Page Range: 4337-4349

Description:

No description provided

Open Access: Yes

DOI: 10.52091/EVIK-2023/1-2-HUN

In vitro evaluation of certain probiotic properties of lactic acid bacteria isolates

Judit Süle Zoltán Hatvan Z. Kerényi László Varga

Publication Name: Elelmiszervizsgalati Kozlemenyek

Publication Date: 2023-01-01

Volume: 69

Issue: 1

Page Range: 4350-4363

Description:

Bacteria carrying genes responsible for antibiotic resistance cannot be used in food production. For this reason, exploring the antibiotic resistance profile of probiotic candidates and the antimicrobial substances they produce are essential for probiotic strain selection. The aim of this study was to develop and evaluate additional elements of a complex in vitro test system for rapid and efficient selection of a large number of putative probiotic isolates. In a previous work, we had tested bacterial strains (n=217) isolated from Transylvanian raw sheep milk, cultured sheep milk, and sheep cheese samples and we reduced the sample number to a total of six Gram-positive, non-hemolytic, catalase-negative, well-aggregating, good acid and bile acid tolerating strains. In this research, we investigated the antibiotic resistance and antimicrobial production capacity of the pre-selected strains (n=6). The antimicrobial activity of the isolates was determined by the agar well diffusion assay. Strains E15, E66, E173, E198, and E216 were found to inhibit the growth of both Salmonella Enteridis ATCC 13076 and the control strain (i.e., Lactobacillus acidophilus ATCC 4356). Antibiotic resistance tests were performed by the agar disk diffusion method. All six isolates belonging to the species of Levilactobacillus brevis and Lactiplantibacillus plantarum were found to be resistant to several antibiotics and, therefore, cannot be used for the manufacture of commercial probiotic products. In conclusion, our in vitro test system proved to be capable of effectively screening out unsafe isolates.

Open Access: Yes

DOI: 10.52091/EVIK-2023/1-2-ENG

Developed rapid and simple RP-HPLC method for simultaneous separation and quantification of bovine milk protein fractions and their genetic variants

G. Szafner Solveig Bukovics Judit Süle A. J. Kovács Székelyhidi Rita Katalin Szabó Henrietta Buzás

Publication Name: Analytical Biochemistry

Publication Date: 2022-12-01

Volume: 658

Issue: Unknown

Page Range: Unknown

Description:

The aim was to develop a reliable rapid reversed-phase high-performance liquid chromatography (RP-HPLC) method to simultaneously determine the main bovine milk protein fractions, including their genetic variants. Compared to the previous studies, our method is able to separate the main protein fractions within 20 min of total run time. The method validation consisted of testing repeatability, reproducibility linearity, repeatability, and accuracy. The procedure was developed using raw individual, bulk, and commercially available heat-treated cow milk samples. The RSD of peak areas ranged from 1.43 to 3.16% within analytical day and from 3.29 to 6.70% across analytical days. The method can be applied to investigate both raw and heat-treated milk samples.

Open Access: Yes

DOI: 10.1016/j.ab.2022.114939

In vitro tesztrendszer alkalmazása probiotikus baktériumtörzsek szelektálására

Judit Süle Zoltán Hatvan Z. Kerényi László Varga

Publication Name: Elelmiszervizsgalati Kozlemenyek

Publication Date: 2022-06-30

Volume: 68

Issue: 2

Page Range: 3904-3915

Description:

No description provided

Open Access: Yes

DOI: 10.52091/EVIK-2022/2-4-ENG