Sárvári Loretta Csilla

58115084100

Publications - 7

Quantitative comparison of some faecal bacterial communities in groups of Mangalica and commercial pigs

Károly Tempfli László Pongrácz Klaudia Szalai Sárvári Loretta Csilla Emil Balázs Herceg Ágnes Bali Papp

Publication Name: Bio Web of Conferences

Publication Date: 2024-08-23

Volume: 125

Issue: Unknown

Page Range: Unknown

Description:

Different housing technology, breed, age and nutrition can contribute to changes in the composition of microbial communities in pigs. Faecal samples from groups of Mangalica and commercial pigs were collected and analysed by qPCR in order to identify changes and differences regarding the quantity of total faecal bacteria, Prevotella genus, Lactobacillus spp., Bifidobacterium spp., Enterococcus spp. and the family Enterobacteriaceae. In both Mangalica and commercial pig samples, quantities of total faecal bacteria increased from weaner pigs to lactating sows. The relative quantity of total bacteria was larger (p<0.05) in Mangalica growers and lactating sows compared to commercial pigs. The ratio of Prevotella genus in total bacteria was higher (p<0.05) in Mangalica growers and lower in Mangalica lactating sows compared to respective commercial groups. The ratio of Lactobacillus spp. was largest (p<0.05) in samples of Mangalica boars, whereas ratios of Bifidobacterium spp. were greater (p<0.05) in Mangalica weaners, growers, and boars. Faecal samples of Mangalica growers contained a higher ratio of Enterobacteriaceae in total bacteria, whereas Enterococcus spp. was more prevalent in commercial weaner pigs and boars (p<0.05). Considerable changes in faecal bacteria communities were observed in association with different age and utilization.

Open Access: Yes

DOI: 10.1051/bioconf/202412503005

Effects of a Probiotic Supplement on the Quantity of Some Bacterial Communities in Fecal Samples of Lactating Sows

Tamás Tóth Viktor J. Vojnich Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Sárvári Loretta Csilla Botond Alpár

Publication Name: Chemical Engineering Transactions

Publication Date: 2024-01-01

Volume: 114

Issue: Unknown

Page Range: 949-954

Description:

Complex adaptation strategies concerning nutrition, housing technology, and veterinary treatment are required to maintain current production levels under increasingly stringent regulations on the preventive application of antibiotics. The reduced application of antibiotics is recommended for the sustainability of industrial pig production. Probiotic supplementation may contribute to improved sow and piglet health, mitigating the need for antibiotics. The effects of probiotic supplementation on sow performance and the quantity of fecal bacterial communities in lactating sows were investigated. Experimental sows received probiotic supplementation (n=10) and were compared to control sows (n=10). Fecal samples were collected from 20 sows in the second week of lactation. The quantitative measurement of total bacteria, Prevotella genus, Lactobacillus spp., and Bifidobacterium spp. was done by qPCR. Differences in backfat thickness (BFT), BFT loss, and feed intake of control and experimental sows were not significant (p>0.05). The amount of total bacteria, Prevotella, and Lactobacillus spp. was lower (p<0.05) in the fecal samples of experimental sows. The Prevotella percentage in total bacteria decreased, whereas Bifidobacterium spp. the ratio increased in experimental supplemented sows. Overall, probiotic supplementation resulted in notable alterations regarding some of the analyzed bacterial communities.

Open Access: Yes

DOI: 10.3303/CET24114159

Aflatoxin M1 detection in raw milk and drinking milk in Hungary by ELISA − A one-year survey

Attila Hucker Róbert Kocsis G. Szafner Solveig Bukovics Judit Süle A. J. Kovács Katalin Szabó Henrietta Buzás Sárvári Loretta Csilla Katalin Nagy-Kovács

Publication Name: Journal of Food Composition and Analysis

Publication Date: 2023-08-01

Volume: 121

Issue: Unknown

Page Range: Unknown

Description:

The aim of this study was to monitor the aflatoxin M1(AFM1) contamination in raw milk and drinking milk in Hungary over a one-year period. A total of 474 milk samples of raw milk (n = 278) and commercial milk (n = 196) were collected and analysed between September 2021 and November 2022. Enzyme-Linked Immunosorbent Assay (ELISA) determined the concentration of AFM1. It was found that 68.7% (191/278) of the raw cow milk samples were contaminated by AFM1 in the range 5.0–173 ng/L, the mean of the positive samples was 30.7 ± 24.7 ng/L, and the median was 21.8 ng/L. The percentage of contamination in drinking milk was 79.1% (155/196). The mean, median, and range of the positive samples were 18.0 ± 10.9 ng/L, 16.18 ng/L, and 5.3–100 ng/L, respectively. Overall, 9.4% (26/278) of raw milk samples and only 1 commercial milk sample of 196 (0.5%) contained AFM1 exceeding the maximum residue level (MRL) of 50 ng/L set by the European Union. Our study suggests that based on calculated AFM1-related health risk indicators, the Hungarian adult population are not exposed to high levels of AFM1, but regular monitoring of aflatoxins is necessary not only for dairy farmers but also for the milk processing sectors.

Open Access: Yes

DOI: 10.1016/j.jfca.2023.105368

Gene Expression of Peroxisome Proliferator-Activated Receptor γ in Mangalica and Large White Pigs

Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Sárvári Loretta Csilla Éva Rampasek

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 271-276

Description:

The expression of peroxisome proliferator-activated receptor γ gene (PPARγ) – a major regulator of adipogenesis and intramuscular fat deposition in pigs – was analysed in different tissues of Blonde Mangalica (M) and Hungarian Large White (LW) by means of quantitative reverse transcription PCR. In total, 20 experimental animals were raised and fed under identical conditions, and transported to the abattoir at similar body weight (124.7±10.3 kg; p>0.05). In both breeds highest PPARγ expression was observed in backfat (p<0.001), whereas lower levels were measured in muscle samples. The PPARγ mRNA levels in loin (m. longissimus dorsi) and semimembranosus muscle samples did not differ (p>0.05). Elevated PPARγ expression was observed in M backfat compared to LW (p<0.05). Loin and semimembranosus muscle samples of M showed higher PPARγ mRNA levels than LW (P<0.001), as well. Loin muscle marbling scores significantly correlated with β-actin normalized PPARγ expression (r=0.63; p<0.001) indicating the involvement of PPARγ in the regulation of intramuscular fat development. In conclusion, the differential expression of PPARγ in backfat and muscle samples can contribute to the remarkable differences in the constitution and intramuscular fat accumulation of fat-type M and lean-type commercial LW. Muscle PPARγ mRNA levels may be used as an indicator of marbling characteristics in pigs.

Open Access: Yes

DOI: 10.3303/CET23107046

Yield Performance, Laying Behaviour Traits and Egg Quality of a Crossbred Laying Hen in Alternative Housing Systems

Eszter Zsédely Károly Tempfli Klaudia Szalai Erika Lencsés-Varga G. Takács Sárvári Loretta Csilla

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 121-126

Description:

One aim of this study was to investigate a crossbred laying hen line (400 hens) in two alternative housing systems in two replicates between 20-72 weeks of age. One rearing system was deep litter (D) with artificial light. Hens from Group R were housed in a poultry house with windows on deep litter and access to a runway without artificial light. Birds were fed ad libitum with a concentrate layer feed (18.0 % protein, 11.6 MJ). The egg production was influenced by the housing system. Lower average egg weight was observed in Group R. The feed consumption was also lower here, but the FCR was better compared to D. Floor egg did not cause any problem in the examined systems. The shell strength decreased with age in both systems. However, the rate was higher in Group D. The albumen height and HU value were higher in Group R at the end of the experiment (72nd week of age). Some differences were found in the fatty acid profile between the two housing systems. Another goal of the study was to observe the behaviour patterns of animals kept under different conditions. Behavioural traits were monitored for 100 hens in total on the two farms that applied different housing technologies (50-50 birds with or without outdoor access). Birds with outdoor access showed 0.23±0.81 aggression-related traits per hour, whereas chickens without outdoor access produced 0.15±0.76 of the same traits (p>0.05). Hens with outdoor access showed 2.30±4.65 activity-related traits, whereas, for hens without outdoor access, 0.72±1.83 observations were recorded per hour (p<0.05). Comfort behaviour was similar (p>0.05) in the two groups. In conclusion, the housing system has a complex effect on the assessed crossbred laying hen line.

Open Access: Yes

DOI: 10.3303/CET23107021

Beta-Casein Genotyping in Dairy Cow Herds in Győr-Moson-Sopron County

Károly Tempfli Henrietta Buzás Sárvári Loretta Csilla Zsofia Meszaros László Gulyás

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 451-456

Description:

The main objective of this study was to determine the beta-casein A1/A2 polymorphism status of animals in two Holstein Friesian dairy cow herds in Győr-Moson-Sopron County, Hungary. The A1/A2 status of cattle is determined by the beta-casein gene on the sixth chromosome. The analysed single nucleotide polymorphism is non-synonymous; A1 and A2 variants of bovine beta-casein differ at position 67 of the amino acid chain: A1 variant codes for histidine and A2 codes for proline, which may affect the milk protein degradation process. The analysed polymorphism leads to key conformational changes in the secondary protein structure of beta-casein. Beta-casomorphin (known as BCM7) is released only from A1-type milk and cannot be completely degraded by enzymes during digestion. DNA isolation was performed from whole blood, and a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with agarose gel electrophoresis was applied in order to determine individual genotypes. The results from the two dairy farms demonstrate that a high proportion of cows (86.08 and 90.74 %) carry the A2 gene variant without targeted selection. At farm „A”, beta-casein polymorphisms were determined in 599 cows and 148 heifers. The genotype distribution of the cows was 47.25 % heterozygous, 38.83 % homozygous A2, whereas 14.02 % of the cows carried the A1A1 genotype. In heifers, A2A2 was already present in a remarkably high frequency (91.89 %), whereas the prevalence of heterozygotes was 7.43 %, and A1A1 animals made up only 0.67 % of the analysed heifer population. In Hungary, a growing number of dairy farms are using verified A2 homozygous breeding bull semen. The introduction of homozygous A2 sperm on the farm „A” remarkably increased A2 frequency in the heifer population. In total, 324 cows were genotyped on farm „B”, where the A2A2 genotype was observed in 30.55 % of the animals. The second most common genotype was A1A2, with a genotype frequency of 60.19 %, whereas A1A1 homozygotes occurred with a 9.26 % frequency. The growing popularity of A2 milk due to potential health benefits is driving Hungarian stakeholders towards the targeted selection of dairy populations; animal genotyping is an evident approach to facilitate this transition.

Open Access: Yes

DOI: 10.3303/CET23107076

Genotyping of a SORCS2 polymorphism and behavioural trait associations in newly developed laying hen populations

Eszter Zsédely Károly Tempfli Hidas András Erika Lencsés-Varga Szálai Klaudia Almási Anita Orbán Attila Sárvári Loretta Csilla Ágnes Bali Papp

Publication Name: Magyar Allatorvosok Lapja

Publication Date: 2021-06-01

Volume: 143

Issue: 6

Page Range: 351-358

Description:

Background: Cage-free egg production methods are advancing in the European Union due to stringent housing regulations, animal welfare and consumer pressure. A changing production environment requests novel adaptation strategies of which the development of specific layer hybrids or strains is notably promising. Cage-free housing systems allow for a rather complete expression of social behaviour in laying hen populations; however, increasing stress, aggression and feather pecking are considered as major consequences. Objectives: This study aimed to detect a recently described SORCS2 polymorphism in newly developed Hungarian laying hen populations and observe potential associations with behavioural traits. Cage-free housing technologies with or without outdoor acces were also compared. Materials and Methods: Occurrence of behavioural traits were individually recorded for 119 hens. A novel PCR-RFLP method was developed and applied for SORCS2 genotyping with Rsal endonuclase. Results and Discussion: The SORCS2 polymorphism was present In the Hungarian populations, Callele frequencies varied between 75.1-85.5%. Homozygous TT genotype was the least prevalent across the three experimental populations with frequencies 2.2-4.2%. The experimental hybrid populations demonstrated different (p < 0.05) levels of aggression and activity related behavioural patterns. Homozygous CC animals showed more cases of aggressive behaviour compared to other genotypes. Feather pecking was more common In populations with outdoor access compared to the closed barn system (p < 0.05). The social behaviour of laying hens Is affected by many factors in an Immensely complex system. Selection efficiency on behaviour can be improved with a more profound knowledge on Its genetics.

Open Access: Yes

DOI: DOI not available