Ágnes Bali Papp

8233762300

Publications - 5

Genotyping of a SORCS2 polymorphism and behavioural trait associations in newly developed laying hen populations

Eszter Zsédely Károly Tempfli Hidas András Erika Lencsés-Varga Szálai Klaudia Almási Anita Orbán Attila Sárvári Loretta Csilla Ágnes Bali Papp

Publication Name: Magyar Allatorvosok Lapja

Publication Date: 2021-06-01

Volume: 143

Issue: 6

Page Range: 351-358

Description:

Background: Cage-free egg production methods are advancing in the European Union due to stringent housing regulations, animal welfare and consumer pressure. A changing production environment requests novel adaptation strategies of which the development of specific layer hybrids or strains is notably promising. Cage-free housing systems allow for a rather complete expression of social behaviour in laying hen populations; however, increasing stress, aggression and feather pecking are considered as major consequences. Objectives: This study aimed to detect a recently described SORCS2 polymorphism in newly developed Hungarian laying hen populations and observe potential associations with behavioural traits. Cage-free housing technologies with or without outdoor acces were also compared. Materials and Methods: Occurrence of behavioural traits were individually recorded for 119 hens. A novel PCR-RFLP method was developed and applied for SORCS2 genotyping with Rsal endonuclase. Results and Discussion: The SORCS2 polymorphism was present In the Hungarian populations, Callele frequencies varied between 75.1-85.5%. Homozygous TT genotype was the least prevalent across the three experimental populations with frequencies 2.2-4.2%. The experimental hybrid populations demonstrated different (p < 0.05) levels of aggression and activity related behavioural patterns. Homozygous CC animals showed more cases of aggressive behaviour compared to other genotypes. Feather pecking was more common In populations with outdoor access compared to the closed barn system (p < 0.05). The social behaviour of laying hens Is affected by many factors in an Immensely complex system. Selection efficiency on behaviour can be improved with a more profound knowledge on Its genetics.

Open Access: Yes

DOI: DOI not available

Str diversity of a historical sheep breed bottlenecked, the cikta

Petra Zenke Ákos Maróti-Agóts László Sáfár Károly Tempfli Endre Kovács A. Shannon András Gáspárdy Ágnes Bali Papp

Publication Name: Journal of Animal and Plant Sciences

Publication Date: 2019-02-01

Volume: 29

Issue: 1

Page Range: 41-47

Description:

The population structure of the endangered Cikta sheep breed was evaluated by means of nine microsatellite polymorphisms. Seventy-two individuals from three flocks were sampled to determine genetic indices in the Hungarian population. Overall, average observed and effective allele numbers were 5.63 and 3.76, respectively. Discriminant analysis based on genotype frequencies revealed moderate genetic diversity among Cikta flocks, since only three loci (OarCP49, CSSM47 and OarHH41) contributed significantly (P<0.05) to differences between subpopulations. Low squared Mahalanobis distances from group centroids also confirmed that the breed is almost equally represented by the three flocks. Moderate level of diversity between flocks was attributed to the long-term effects of a population bottleneck dating back to the 1970s. Negative average FIS value (-0.18) indicated heterozygote excess. Chi-squared tests identified significant (P<0.05) deviation from HWE in the case of BM8125, CSSM47, and MAF214 markers. Continuous microsatellite information is required for the preservation of rare alleles and diversity in Cikta sheep.

Open Access: Yes

DOI: DOI not available

Genotyping of four loci in Hungarian yellow and broiler chickens

Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Ágnes Bali Papp

Publication Name: Acta Veterinaria Hungarica

Publication Date: 2019-01-01

Volume: 67

Issue: 1

Page Range: 1-10

Description:

Traditional selection has led to remarkable differences in allele frequencies among various chicken breeds. Indigenous and broiler-type chicken populations were genotyped for polymorphisms in thyroid hormone responsive Spot14α, prolactin (PRL), IGF-binding protein 2 (IGFBP2), and somatostatin (SST) genes in order to determine potential utilisation type-associated allele frequencies. Significant (P < 0.05) differences were detected between Hungarian Yellow and broiler populations for Spot14α, PRL, and IGFBP2 allele frequencies, whereas the same SST allele (A) was fixed in both groups. In this study, the most significant associations (P < 0.05) were found between the IGFBP2 genotypes and the measured traits (body weight, carcass weight, breast muscle weight with or without skin, breast muscle weight as a percentage of carcass weight) in the broiler population. The results can be applied for the evaluation of polymorphism effects in the analysed populations; however, contradictory allele effects in different breeds and hybrids indicate the need for cautious marker utilisation in selection programmes.

Open Access: Yes

DOI: 10.1556/004.2019.001

The response of prion genic variation to selection for scrapie resistance in Hungarian indigenous sheep breeds

Petra Zenke Ákos Maróti-Agóts László Sáfár Endre Kovács Viktoria Holly András Gáspárdy Ágnes Bali Papp

Publication Name: Acta Veterinaria Hungarica

Publication Date: 2018-01-01

Volume: 66

Issue: 4

Page Range: 562-572

Description:

The authors studied the present status of Hungarian indigenous sheep breeds based on the genetic background of scrapie resistance. The aim of this investigation was to estimate the relative frequency of prion haplotypes, genotypes and risk categories, as well as to reveal the efficiency of the scrapie eradication programme achieved over the last decade. A novel approach in the characterisation of prion by using its genic variation was also implemented. The authors established that the proportion of deleterious sites (%) can be a useful indicator of the eradication programme. Based on a large sample size, it was confirmed that the scrapie resistance of the Cikta breed is low, and the classification of this breed according to risk category has not improved. However, the frequent genotype ARQ and risk category 3 can also be considered characteristic of the breed. The careful use of these genotypes is permitted and will contribute to the maintenance of breed diversity. The response of prion genic variation to selection for scrapie resistance in the other breeds (Tsigai, Milking Tsigai, White Racka, Black Racka and Gyimes Racka) was definitely successful.

Open Access: Yes

DOI: 10.1556/004.2018.050

Differential expression of six genes in fat-type Hungarian Mangalica and other pigs

Zoltán Simon Károly Tempfli László Pongrácz Barbara Kiss Klaudia Szalai Ágnes Bali Papp

Publication Name: Archives Animal Breeding

Publication Date: 2016-06-14

Volume: 59

Issue: 2

Page Range: 259-265

Description:

In order to identify potential variances in gene expression of phenotypically different pig breeds, six fat-metabolism-related genes were analyzed in backfat and muscle tissues of fat-type Mangalica (MAN), Mangalica x Duroc (MD), and lean-type Hungarian Large White (HLW) and Pietrain x Duroc (PD) pigs by means of quantitative reverse transcription PCR (qRT-PCR). Higher (P < 0.05) adipocyte fatty-acid-binding protein (A-FABP) expression was observed in backfat and muscle tissues of purebred and crossbred MAN than in those of HLW and PD. In all breeds and crosses, adiponectin (ADIPOQ) was predominantly expressed in backfat at a similar level (P > 0.05), whereas muscle ADIPOQ expression was highest (P < 0.05) in MAN and MD. Levels of fatty acid synthase (FASN) mRNA were greatest in MAN, moderate in MD, and lowest in HLW and PD backfat and muscle. The fat mass and obesity-associated gene (FTO) was more abundant in MAN and MD backfat, whereas muscle expressions did not differ (P > 0.05) between breeds. Regarding leptin (LEP) expression, MAN produced the greatest levels in backfat, while HLW produced the lowest. In muscle, highest LEP was detected in MAN and MD. Between groups, perilipin 2 (PLIN2) was expressed similarly in backfat; however, PLIN2 was more abundant in muscle of MAN and MD than in that of HLW and PD. Differences in gene expression can contribute to the development of the characteristic fatty phenotype in MAN pigs. The identification of differentially expressed genes facilitates targeted sequencing and genotyping efforts for further studies.

Open Access: Yes

DOI: 10.5194/aab-59-259-2016