Károly Tempfli

53464277300

Publications - 14

Effect of some myostatin (MSTN) variants on live weight and beef traits measured by ultrasound in Charolais candidate breeding bulls

Márton Török Szabolcs Bene Gabriella Holló Károly Tempfli Edit Mikó Tamás Csürhés F. Szabó

Publication Name: Journal of Applied Animal Research

Publication Date: 2025-01-01

Volume: 53

Issue: 1

Page Range: Unknown

Description:

This study investigated the relationship between some ultrasound measurements, live weight data, and five (F94L, nt267, nt324, nt414 and Q204X) myostatin (MSTN) variants associated with beef production in Charolais cattle. A total of 390 Charolais candidate breeding bulls were weighed at birth (BIW) as well as 205 (CWW) and 400 days (FDW) of age, and ultrasonically measured for ribeye area (REA), fat thickness at rump (FRU), fat thickness at ribeye (FRI) and marbling of the ribeye (MAR) at 400 days of age. The frequency of allele C and A were high in the studied population. The F94L had a significant effect on the FRU and FRI, whereas REA significant differed between homozygous and heterozygous animals on SNP at nt267. MAR of non-mutant animals were significantly (by 04.−05 points) lower than those heterozygous for the F94L. FRI tended to be higher (0.51–0.57 cm) for mutations together nt267 and nt414, or for F94L, nt414 and nt324, but lower (0.47–0.49 cm) for nt267 and nt414 mutations. The results indicating F94L could be use in QTL selection for improving beef quality. The polygenetic effect of different MSTN mutations, as well as the effect of MSTN deficiency, would be worth investigating in the future.

Open Access: Yes

DOI: 10.1080/09712119.2025.2452470

Quantitative comparison of some faecal bacterial communities in groups of Mangalica and commercial pigs

Károly Tempfli László Pongrácz Klaudia Szalai Sárvári Loretta Csilla Emil Balázs Herceg Ágnes Bali Papp

Publication Name: Bio Web of Conferences

Publication Date: 2024-08-23

Volume: 125

Issue: Unknown

Page Range: Unknown

Description:

Different housing technology, breed, age and nutrition can contribute to changes in the composition of microbial communities in pigs. Faecal samples from groups of Mangalica and commercial pigs were collected and analysed by qPCR in order to identify changes and differences regarding the quantity of total faecal bacteria, Prevotella genus, Lactobacillus spp., Bifidobacterium spp., Enterococcus spp. and the family Enterobacteriaceae. In both Mangalica and commercial pig samples, quantities of total faecal bacteria increased from weaner pigs to lactating sows. The relative quantity of total bacteria was larger (p<0.05) in Mangalica growers and lactating sows compared to commercial pigs. The ratio of Prevotella genus in total bacteria was higher (p<0.05) in Mangalica growers and lower in Mangalica lactating sows compared to respective commercial groups. The ratio of Lactobacillus spp. was largest (p<0.05) in samples of Mangalica boars, whereas ratios of Bifidobacterium spp. were greater (p<0.05) in Mangalica weaners, growers, and boars. Faecal samples of Mangalica growers contained a higher ratio of Enterobacteriaceae in total bacteria, whereas Enterococcus spp. was more prevalent in commercial weaner pigs and boars (p<0.05). Considerable changes in faecal bacteria communities were observed in association with different age and utilization.

Open Access: Yes

DOI: 10.1051/bioconf/202412503005

Effects of a Probiotic Supplement on the Quantity of Some Bacterial Communities in Fecal Samples of Lactating Sows

Tamás Tóth Viktor J. Vojnich Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Sárvári Loretta Csilla Botond Alpár

Publication Name: Chemical Engineering Transactions

Publication Date: 2024-01-01

Volume: 114

Issue: Unknown

Page Range: 949-954

Description:

Complex adaptation strategies concerning nutrition, housing technology, and veterinary treatment are required to maintain current production levels under increasingly stringent regulations on the preventive application of antibiotics. The reduced application of antibiotics is recommended for the sustainability of industrial pig production. Probiotic supplementation may contribute to improved sow and piglet health, mitigating the need for antibiotics. The effects of probiotic supplementation on sow performance and the quantity of fecal bacterial communities in lactating sows were investigated. Experimental sows received probiotic supplementation (n=10) and were compared to control sows (n=10). Fecal samples were collected from 20 sows in the second week of lactation. The quantitative measurement of total bacteria, Prevotella genus, Lactobacillus spp., and Bifidobacterium spp. was done by qPCR. Differences in backfat thickness (BFT), BFT loss, and feed intake of control and experimental sows were not significant (p>0.05). The amount of total bacteria, Prevotella, and Lactobacillus spp. was lower (p<0.05) in the fecal samples of experimental sows. The Prevotella percentage in total bacteria decreased, whereas Bifidobacterium spp. the ratio increased in experimental supplemented sows. Overall, probiotic supplementation resulted in notable alterations regarding some of the analyzed bacterial communities.

Open Access: Yes

DOI: 10.3303/CET24114159

Gene Expression of Peroxisome Proliferator-Activated Receptor γ in Mangalica and Large White Pigs

Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Sárvári Loretta Csilla Éva Rampasek

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 271-276

Description:

The expression of peroxisome proliferator-activated receptor γ gene (PPARγ) – a major regulator of adipogenesis and intramuscular fat deposition in pigs – was analysed in different tissues of Blonde Mangalica (M) and Hungarian Large White (LW) by means of quantitative reverse transcription PCR. In total, 20 experimental animals were raised and fed under identical conditions, and transported to the abattoir at similar body weight (124.7±10.3 kg; p>0.05). In both breeds highest PPARγ expression was observed in backfat (p<0.001), whereas lower levels were measured in muscle samples. The PPARγ mRNA levels in loin (m. longissimus dorsi) and semimembranosus muscle samples did not differ (p>0.05). Elevated PPARγ expression was observed in M backfat compared to LW (p<0.05). Loin and semimembranosus muscle samples of M showed higher PPARγ mRNA levels than LW (P<0.001), as well. Loin muscle marbling scores significantly correlated with β-actin normalized PPARγ expression (r=0.63; p<0.001) indicating the involvement of PPARγ in the regulation of intramuscular fat development. In conclusion, the differential expression of PPARγ in backfat and muscle samples can contribute to the remarkable differences in the constitution and intramuscular fat accumulation of fat-type M and lean-type commercial LW. Muscle PPARγ mRNA levels may be used as an indicator of marbling characteristics in pigs.

Open Access: Yes

DOI: 10.3303/CET23107046

Yield Performance, Laying Behaviour Traits and Egg Quality of a Crossbred Laying Hen in Alternative Housing Systems

Eszter Zsédely Károly Tempfli Klaudia Szalai Erika Lencsés-Varga G. Takács Sárvári Loretta Csilla

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 121-126

Description:

One aim of this study was to investigate a crossbred laying hen line (400 hens) in two alternative housing systems in two replicates between 20-72 weeks of age. One rearing system was deep litter (D) with artificial light. Hens from Group R were housed in a poultry house with windows on deep litter and access to a runway without artificial light. Birds were fed ad libitum with a concentrate layer feed (18.0 % protein, 11.6 MJ). The egg production was influenced by the housing system. Lower average egg weight was observed in Group R. The feed consumption was also lower here, but the FCR was better compared to D. Floor egg did not cause any problem in the examined systems. The shell strength decreased with age in both systems. However, the rate was higher in Group D. The albumen height and HU value were higher in Group R at the end of the experiment (72nd week of age). Some differences were found in the fatty acid profile between the two housing systems. Another goal of the study was to observe the behaviour patterns of animals kept under different conditions. Behavioural traits were monitored for 100 hens in total on the two farms that applied different housing technologies (50-50 birds with or without outdoor access). Birds with outdoor access showed 0.23±0.81 aggression-related traits per hour, whereas chickens without outdoor access produced 0.15±0.76 of the same traits (p>0.05). Hens with outdoor access showed 2.30±4.65 activity-related traits, whereas, for hens without outdoor access, 0.72±1.83 observations were recorded per hour (p<0.05). Comfort behaviour was similar (p>0.05) in the two groups. In conclusion, the housing system has a complex effect on the assessed crossbred laying hen line.

Open Access: Yes

DOI: 10.3303/CET23107021

Beta-Casein Genotyping in Dairy Cow Herds in Győr-Moson-Sopron County

Károly Tempfli Henrietta Buzás Sárvári Loretta Csilla Zsofia Meszaros László Gulyás

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 451-456

Description:

The main objective of this study was to determine the beta-casein A1/A2 polymorphism status of animals in two Holstein Friesian dairy cow herds in Győr-Moson-Sopron County, Hungary. The A1/A2 status of cattle is determined by the beta-casein gene on the sixth chromosome. The analysed single nucleotide polymorphism is non-synonymous; A1 and A2 variants of bovine beta-casein differ at position 67 of the amino acid chain: A1 variant codes for histidine and A2 codes for proline, which may affect the milk protein degradation process. The analysed polymorphism leads to key conformational changes in the secondary protein structure of beta-casein. Beta-casomorphin (known as BCM7) is released only from A1-type milk and cannot be completely degraded by enzymes during digestion. DNA isolation was performed from whole blood, and a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method with agarose gel electrophoresis was applied in order to determine individual genotypes. The results from the two dairy farms demonstrate that a high proportion of cows (86.08 and 90.74 %) carry the A2 gene variant without targeted selection. At farm „A”, beta-casein polymorphisms were determined in 599 cows and 148 heifers. The genotype distribution of the cows was 47.25 % heterozygous, 38.83 % homozygous A2, whereas 14.02 % of the cows carried the A1A1 genotype. In heifers, A2A2 was already present in a remarkably high frequency (91.89 %), whereas the prevalence of heterozygotes was 7.43 %, and A1A1 animals made up only 0.67 % of the analysed heifer population. In Hungary, a growing number of dairy farms are using verified A2 homozygous breeding bull semen. The introduction of homozygous A2 sperm on the farm „A” remarkably increased A2 frequency in the heifer population. In total, 324 cows were genotyped on farm „B”, where the A2A2 genotype was observed in 30.55 % of the animals. The second most common genotype was A1A2, with a genotype frequency of 60.19 %, whereas A1A1 homozygotes occurred with a 9.26 % frequency. The growing popularity of A2 milk due to potential health benefits is driving Hungarian stakeholders towards the targeted selection of dairy populations; animal genotyping is an evident approach to facilitate this transition.

Open Access: Yes

DOI: 10.3303/CET23107076

Pollen Concentration of Ailanthus Altissima on the South Transdanubia Region in Hungary

Viktor J. Vojnich Károly Tempfli Donát Magyar Árpád Ferencz Tamás Tóth

Publication Name: Lecture Notes in Civil Engineering

Publication Date: 2023-01-01

Volume: 289

Issue: Unknown

Page Range: 265-274

Description:

In areas where the tree of heaven (Ailanthus altissima) appears and multiplies, the original vegetation degrades and transforms. The invasive tree of heaven is also of great importance in urban environments, where it causes building damage, static problems and endangers utilities. Ailanthus pollen concentration was measured during the 3-year period 2018–2020 at three county capitals (Kaposvár, Szekszárd, Pécs) of the South Transdanubia region, Hungary (Somogy county, Tolna county and Baranya county), with a 7-day Hirst-type (Burkard) pollen trap. The extent of the prevalence of A. altissima can be deduced from its pollen concentration. For this purpose, multi-year pollen data are displayed on a result map in which areas characterized by different pollen concentrations are represented by colour codes. The mass appearance of A. altissima is a serious problem in almost all areas (national parks, forests, inner city zones and towns), where its control/eradication would cost millions of euros. Pollen monitoring provides information on the size of A. altissima stands and provide a basis for proposals and plans for measures to control this invasive tree species and mitigate the damage caused by it.

Open Access: Yes

DOI: 10.1007/978-3-031-13090-8_27

Genotyping of a SORCS2 polymorphism and behavioural trait associations in newly developed laying hen populations

Eszter Zsédely Károly Tempfli Hidas András Erika Lencsés-Varga Szálai Klaudia Almási Anita Orbán Attila Sárvári Loretta Csilla Ágnes Bali Papp

Publication Name: Magyar Allatorvosok Lapja

Publication Date: 2021-06-01

Volume: 143

Issue: 6

Page Range: 351-358

Description:

Background: Cage-free egg production methods are advancing in the European Union due to stringent housing regulations, animal welfare and consumer pressure. A changing production environment requests novel adaptation strategies of which the development of specific layer hybrids or strains is notably promising. Cage-free housing systems allow for a rather complete expression of social behaviour in laying hen populations; however, increasing stress, aggression and feather pecking are considered as major consequences. Objectives: This study aimed to detect a recently described SORCS2 polymorphism in newly developed Hungarian laying hen populations and observe potential associations with behavioural traits. Cage-free housing technologies with or without outdoor acces were also compared. Materials and Methods: Occurrence of behavioural traits were individually recorded for 119 hens. A novel PCR-RFLP method was developed and applied for SORCS2 genotyping with Rsal endonuclase. Results and Discussion: The SORCS2 polymorphism was present In the Hungarian populations, Callele frequencies varied between 75.1-85.5%. Homozygous TT genotype was the least prevalent across the three experimental populations with frequencies 2.2-4.2%. The experimental hybrid populations demonstrated different (p < 0.05) levels of aggression and activity related behavioural patterns. Homozygous CC animals showed more cases of aggressive behaviour compared to other genotypes. Feather pecking was more common In populations with outdoor access compared to the closed barn system (p < 0.05). The social behaviour of laying hens Is affected by many factors in an Immensely complex system. Selection efficiency on behaviour can be improved with a more profound knowledge on Its genetics.

Open Access: Yes

DOI: DOI not available

Linseed oil supplementation affects fatty acid desaturase 2, peroxisome proliferator activated receptor gamma, and insulin-like growth factor 1 gene expression in turkeys (Meleagris gallopavo)

Eszter Zsédely E. Lakatos Károly Tempfli Klaudia Szalai András Gáspárdy Ágnes Bali Papp

Publication Name: Animal Bioscience

Publication Date: 2021-04-01

Volume: 34

Issue: 4

Page Range: 662-669

Description:

Objective: Effects of linseed oil (LO) supplementation on the fat content and fatty acid profile of breast meat, and the expression of three genes in the liver, breast muscle and fat tissues of commercial 154-day-old hybrid male turkeys were investigated. Methods: The animals in the control group were fed a commercially available feed and received no LO supplementation (n = 70), whereas animals in the LO group (n = 70) were fed the same basic diet supplemented with LO (day 15 to 21, 0.5%; day 22 to 112, 1%). The effect of dietary LO supplementation on fatty acid composition of breast muscle was examined by gas chromatography, and the expression of fatty acid desaturase 2 (FADS2), peroxisome proliferator activated receptor gamma (PPARγ), and insulin-like growth factor 1 (IGF1) genes was analysed by means of quantitative reverse transcription polymerase chain reaction. Results: The LO supplementation affected the fatty acid composition of breast muscle. Hepatic FADS2 levels were considerably lower (p<0.001), while adipose tissue expression was higher (p<0.05) in the control compared to the LO group. The PPARγ expression was lower (p<0.05), whereas IGF1 was higher (p<0.05) in the fat of control animals. There were no significant (p>0.05) differences in FADS2, PPARγ, and IGF1 gene expressions of breast muscle; however, omega-6/omega-3 ratio of breast muscle substantially decreased (p<0.001) in the LO group compared to control. Conclusion: Fatty acid composition of breast meat was positively influenced by LO supplementation without deterioration of fattening parameters. Remarkably, increased FADS2 expression in the liver of LO supplemented animals was associated with a significantly decreased omega-6/omega-3 ratio, providing a potentially healthier meat product for human consumption. Increased PPARγ expression in fat tissue of the LO group was not associated with fat content of muscle, whereas a decreased IGF1 expression in fat tissue was associated with a trend of decreasing fat content in muscle of the experimental LO group.

Open Access: Yes

DOI: 10.5713/ajas.20.0030

Characterisation of Hungarian Cikta sheep based on the control region of mtDNA

Petra Zenke Ákos Maróti-Agóts Károly Tempfli Annus Kata Sáfár László Kovács Endre Harmat Levente András Gáspárdy

Publication Name: Magyar Allatorvosok Lapja

Publication Date: 2020-07-01

Volume: 142

Issue: 7

Page Range: 421-428

Description:

Background: The consideration of the high genetic diversity is indispensable on the course of preservation of endangered animal breeds. Objectives: The authors evaluate the genetic background in the Hungarian native Cikta breed by use of mitochondrial DNA (mtDNA) control region (CR) sequence firstly. Their investigation was carried out in order to serve data for the maintenance of maternal lineages. Materials and Methods: The DNA samples were taken from the descendants of the eldest families by use of founder sampling method based on pedigree (n = 69) in 2015. The primers described by Hiendleder et al. (7) were used to amplify the region of interest (AF010406). Results and Discussion: The control region of mtDNA showed polymorphisms at 32 sites. However, the herds shared 24 polymorphic sites, so the maternal background of the Cikta appears to be genetically uniform. The total number of haplotypes were 13, furthermore, most of the samples belonged to the haplog-roup B of sheep. This fact proves the decisively European maternal origin of the Hungarian Cikta. The average number of pairwise differences (k) and the average nucleotide diversity (ro) were 6.863 and 5.95 × 10-3, respectively. The values of the Cikta population were not significant (p < 0.10) neither by the Tajima D-test (0.107) and by Fu's Fs statistics (2.533), meaning that the greatly reduced population size of the breed known from the breed history did not cause genetic drift, it is in genetic equilibrium regarding its ancient families. The genetic information confirmed the origin of the families/flocks known from the breed history. A more intense focusing on the maternal side is motivated also by the fact that the females are present at greater number than the males, respectively they remain in breeding for a longer period of time, so they can at larger extent be the depositaries of realization and maintenance of genetic diversity.

Open Access: Yes

DOI: DOI not available

Str diversity of a historical sheep breed bottlenecked, the cikta

Petra Zenke Ákos Maróti-Agóts László Sáfár Károly Tempfli Endre Kovács A. Shannon András Gáspárdy Ágnes Bali Papp

Publication Name: Journal of Animal and Plant Sciences

Publication Date: 2019-02-01

Volume: 29

Issue: 1

Page Range: 41-47

Description:

The population structure of the endangered Cikta sheep breed was evaluated by means of nine microsatellite polymorphisms. Seventy-two individuals from three flocks were sampled to determine genetic indices in the Hungarian population. Overall, average observed and effective allele numbers were 5.63 and 3.76, respectively. Discriminant analysis based on genotype frequencies revealed moderate genetic diversity among Cikta flocks, since only three loci (OarCP49, CSSM47 and OarHH41) contributed significantly (P<0.05) to differences between subpopulations. Low squared Mahalanobis distances from group centroids also confirmed that the breed is almost equally represented by the three flocks. Moderate level of diversity between flocks was attributed to the long-term effects of a population bottleneck dating back to the 1970s. Negative average FIS value (-0.18) indicated heterozygote excess. Chi-squared tests identified significant (P<0.05) deviation from HWE in the case of BM8125, CSSM47, and MAF214 markers. Continuous microsatellite information is required for the preservation of rare alleles and diversity in Cikta sheep.

Open Access: Yes

DOI: DOI not available

Genotyping of four loci in Hungarian yellow and broiler chickens

Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Ágnes Bali Papp

Publication Name: Acta Veterinaria Hungarica

Publication Date: 2019-01-01

Volume: 67

Issue: 1

Page Range: 1-10

Description:

Traditional selection has led to remarkable differences in allele frequencies among various chicken breeds. Indigenous and broiler-type chicken populations were genotyped for polymorphisms in thyroid hormone responsive Spot14α, prolactin (PRL), IGF-binding protein 2 (IGFBP2), and somatostatin (SST) genes in order to determine potential utilisation type-associated allele frequencies. Significant (P < 0.05) differences were detected between Hungarian Yellow and broiler populations for Spot14α, PRL, and IGFBP2 allele frequencies, whereas the same SST allele (A) was fixed in both groups. In this study, the most significant associations (P < 0.05) were found between the IGFBP2 genotypes and the measured traits (body weight, carcass weight, breast muscle weight with or without skin, breast muscle weight as a percentage of carcass weight) in the broiler population. The results can be applied for the evaluation of polymorphism effects in the analysed populations; however, contradictory allele effects in different breeds and hybrids indicate the need for cautious marker utilisation in selection programmes.

Open Access: Yes

DOI: 10.1556/004.2019.001

A specific selection programme is required in the autochthonous Cikta Sheep which is endangered by own frequent ARQ prion haplotype?

Petra Zenke Ákos Maróti-Agóts László Sáfár Károly Tempfli Endre Kovács Susanne Mitro András Gáspárdy Ágnes Bali Papp

Publication Name: Landbauforschung

Publication Date: 2017-01-01

Volume: 67

Issue: 3-4

Page Range: 141-146

Description:

The authors study the current status of Hungarian Cikta Sheep based on genetic background of scrapie resistance. The aim of this investigation was to estimate the relative frequency of prion haplotypes, -genotypes, and risk categories as well as to reveal the efficiency of scrapie eradication program achieved over the last decade. The authors confirmed based on larger sample size the previous knowledge, that the resistance against scrapie of Cikta breed is considered as low, and the classification of this breed according to risk category has not been improved. However, the frequent genotype ARQ and risk category 3 can also be considered for breed specificity. The careful use of these genotypes is permitted and will contribute to the maintenance of breed diversity according to other aspects.

Open Access: Yes

DOI: 10.3220/LBF1513065159000

Differential expression of six genes in fat-type Hungarian Mangalica and other pigs

Zoltán Simon Károly Tempfli László Pongrácz Barbara Kiss Klaudia Szalai Ágnes Bali Papp

Publication Name: Archives Animal Breeding

Publication Date: 2016-06-14

Volume: 59

Issue: 2

Page Range: 259-265

Description:

In order to identify potential variances in gene expression of phenotypically different pig breeds, six fat-metabolism-related genes were analyzed in backfat and muscle tissues of fat-type Mangalica (MAN), Mangalica x Duroc (MD), and lean-type Hungarian Large White (HLW) and Pietrain x Duroc (PD) pigs by means of quantitative reverse transcription PCR (qRT-PCR). Higher (P < 0.05) adipocyte fatty-acid-binding protein (A-FABP) expression was observed in backfat and muscle tissues of purebred and crossbred MAN than in those of HLW and PD. In all breeds and crosses, adiponectin (ADIPOQ) was predominantly expressed in backfat at a similar level (P > 0.05), whereas muscle ADIPOQ expression was highest (P < 0.05) in MAN and MD. Levels of fatty acid synthase (FASN) mRNA were greatest in MAN, moderate in MD, and lowest in HLW and PD backfat and muscle. The fat mass and obesity-associated gene (FTO) was more abundant in MAN and MD backfat, whereas muscle expressions did not differ (P > 0.05) between breeds. Regarding leptin (LEP) expression, MAN produced the greatest levels in backfat, while HLW produced the lowest. In muscle, highest LEP was detected in MAN and MD. Between groups, perilipin 2 (PLIN2) was expressed similarly in backfat; however, PLIN2 was more abundant in muscle of MAN and MD than in that of HLW and PD. Differences in gene expression can contribute to the development of the characteristic fatty phenotype in MAN pigs. The identification of differentially expressed genes facilitates targeted sequencing and genotyping efforts for further studies.

Open Access: Yes

DOI: 10.5194/aab-59-259-2016