E. Lakatos
36602703100
Publications - 43
Comparison of ultrasound-assisted extraction and modified QuEChERS technique for extraction of hesperidin from lemon peel (Citrus limon L.) as determined by HPLC
Publication Name: Journal of Food Composition and Analysis
Publication Date: 2025-06-01
Volume: 142
Issue: Unknown
Page Range: Unknown
Description:
Hesperidin (HSP) is a bioflavonoid known for its notable health benefits. Recent extraction strategies have aimed to minimize hazardous solvent use and simplify procedures to improve cost-effectiveness. This study compares ultrasound-assisted extraction (UAE) with a modified “quick, easy, cheap, effective, rugged, and safe” (QuEChERS) method for the recovery of HSP. High-performance liquid chromatography with diode array detection (HPLC-DAD) was used for quantification. The modified QuEChERS method resulted in the highest extraction efficiency while significantly reducing processing time. The validated method demonstrated excellent sensitivity (LOQ: 10.0 µg/mL), high accuracy (recovery >93 %), and good precision (RSD <3.4 %), making it a reliable and cost-effective approach for routine HSP analysis in citrus peel. These findings indicate that the modified QuEChERS technique offers a promising alternative to UAE for the rapid and efficient extraction of HSP.
Open Access: Yes
The effect of mint addition on the physicochemical and organoleptic properties of strawberry sorbets
Publication Name: Food Chemistry X
Publication Date: 2025-02-01
Volume: 26
Issue: Unknown
Page Range: Unknown
Description:
The study aimed to produce, analyse, and evaluate the consumer acceptance of a functional peppermint-, and spearmint-enriched (0.5,1,2 %) sorbet, which contains only natural substances in its composition, to meet today's popular health-conscious consumer trends. Regarding acid composition, the sorbets contained malic acid, succinic acid, and citric acid. Sorbets regarded of water-soluble sugars, contained sucrose in the lowest concentration, followed by glucose, and the amount of fructose, which is typical for fruits, was the highest. The sorbets' TPC and TAC contents were determined to be between 510.72 and 743.77 mg GAE/kg, and 906.64 and 1137.67 mg AAE/kg respectively. The average melting rate of the desserts was 0.16–0.22 g/min, and based on consumer acceptance, the control and the samples added with 0.5 % spearmint and peppermint mint were the most favorable. The sorbets containing 1 % and 2 % mint had too intense menthol flavor, thus the enjoyment value of the products was reduced based on consumer feedback.
Open Access: Yes
Using natural deep eutectic solvents for the extraction of antioxidant compounds from cornelian cherry (Cornus mas L.) fruits
Publication Name: Green Analytical Chemistry
Publication Date: 2024-12-01
Volume: 11
Issue: Unknown
Page Range: Unknown
Description:
Traditionally used extraction methods are usually based on toxic and/or flammable solvents. Therefore, currently, increased attention has been focused on environmentally friendly green extraction techniques. We aimed to develop a green extraction procedure by using natural deep eutectic solvents (NADES) to recover antioxidant compounds from cornelian cherry fruits (Cornus mas L.). Natural deep eutectic systems combined with maceration with shaking extraction were tested to recover of antioxidant compounds from cornelian cherry fruits. In this study, four different independent variables, namely solvent type (Glu-CA; Glu-Gly; LA-Glu), water concentration (30, 50, and 70 %; v/v), extraction time (30, 60, 90, and 120 min), and extraction temperature (25, 40, and 50 °C) were investigated. Glucose-glycerin (Glu-Gly; 1:1 M ratio) based solvent provided the best results. In addition, one-factor-at-a-time (OFAT) optimization showed that a temperature of 25 °C, 120 min of extraction time, and water concentration of 50 % (v/v) to be the best extraction parameters for Glu-Gly based NADES extraction. The developed method has been successfully applied for the extraction of antioxidant compounds from 13 different cornelian cherry cultivars. NADES can be used safely to extract phenolic compounds from cornelian cherry fruits compared to conventional extraction solvents (water and 80 % (v/v) ethanol).
Open Access: Yes
Valorization of Pea Pod (Pisum sativum L.) Waste: Application as a Functional Ingredient in Flatbreads
Publication Name: Legume Science
Publication Date: 2024-12-01
Volume: 6
Issue: 4
Page Range: Unknown
Description:
Legumes provide valuable functional properties, serving as rich sources of protein, minerals, dietary fiber, and antioxidant compounds. This study evaluated the nutritional and antioxidant properties of pea pod powder (PPP) as a fortifying ingredient in flatbreads. The results indicate that PPP contains carbohydrates (55%), protein (16.7%), and dietary fiber (13.1%), with significant antioxidant properties (total polyphenol content: 463 mg GAE/100 g, total flavonoid content: 159 mg QE/100 g, DPPH radical scavenging activity: 166 mg AAE/100 g; IC50 : 2.31 mg/mL, chlorophyll a: 17.4 mg/100 g, chlorophyll b: 17.4 mg/100 g, and total chlorophyll: 32.6 mg/100 g). Rutin, chlorogenic acid, and p-coumaric acid were identified and quantified by HPLC-DAD, with concentrations of 15.2, 2.94, and 6.60 mg/100 g, respectively. Flatbreads fortified with PPP at 5%w/w and 10%w/w showed increased thickness, reduced diameter and spread ratio, and enhanced antioxidant properties. However, consumer acceptance significantly declined at the 10%w/w level, likely due to off-flavors that affected sensory appeal.
Open Access: Yes
DOI: 10.1002/leg3.70017
Physicochemical and textural properties of gummy candies prepared with fruit vinegar
Publication Name: Applied Food Research
Publication Date: 2024-12-01
Volume: 4
Issue: 2
Page Range: Unknown
Description:
Foods with additional functional value derived from natural sources are becoming more and more in demand these days, including confectionary products. Therefore, the study aimed to develop a gummy candy recipe, enriched with different fruit vinegars and determine the components' effect on the products' mineral, acid, sugar, antioxidant, and polyphenol content, and texture of the confectionery products with analytical methods. In terms of macroelement content, potassium was present in the largest amount in the tested candies (167.87 and 178.83 mg kg-1) and liquid ingredients (57,71–306.93 mg kg-1). In the case of microelements, iron (4.81, 4.79 mg kg-1) and boron (4.62, 4.49 mg kg-1) were significant in the products, iron is also found in gelatin in significant quantities (3.51 mg kg-1), however, boron came exclusively from fruit-derived components (1.02–9.40 mg kg-1). Among the tested organic acids, malic acid (21.67–46.12 mg g-1), and acetic acid (47.74–50.06 mg g-1) were significant in both the raw materials and products, while the fructose content (2.44–439.35 mg g-1) was prominent among the sugars, but the glucose (75.99–163.44 mg g-1) and sucrose (73.61–157.00 mg g-1) content were also significant. Unfortunately, the polyphenol (1.37–1.46 mg GAE/g) and antioxidant (0.29–0.75 mg AAE/g) content of the products was not significant, maybe because of their heat sensitivity. In terms of the texture of the candies, the two candies prepared with different fruit vinegars had similar textural properties, however, in terms of hardness (3523.10 N/m2), gumminess (3266.25 N/m2), and chewiness (3425.33 N/m2) the gummy candy with apple cider vinegar proved to be better. Unlike many other confectionery products, the sugar content of the developed product is provided only by the used the apple concentrate, it does not contain added sugar or sweeteners. The addition of fruit vinegars has been proven to have a positive effect on the nutritional values of candies. By replacing gelatin with another gelling agent, the product can also be prepared in a vegan form.
Open Access: Yes
Impact of Dehydration Techniques on the Nutritional and Microbial Profiles of Dried Mushrooms
Publication Name: Foods
Publication Date: 2024-10-01
Volume: 13
Issue: 20
Page Range: Unknown
Description:
The global consumption of dried mushrooms has increased worldwide because of their rich nutritional value and culinary versatility. Dehydration methods such as sun drying, hot air drying, freeze drying, and microwave drying are employed to prolong the shelf life of a food product. These methods can also affect the food product’s nutritional value and the final product’s microbial profile. Each technique affects the retention of essential nutrients like vitamins, minerals, and bioactive compounds differently. Additionally, these techniques vary in their effectiveness at reducing microbial load, impacting the dried mushrooms’ safety and shelf life. This review addresses the gap in understanding how different dehydration methods influence dried mushrooms’ nutritional quality and microbial safety, which is crucial for optimizing their processing and consumption. It targets researchers, food processors, and consumers seeking to improve the quality and safety of dried mushrooms. This review comprehensively examines the impact of major dehydration techniques, including sun drying, hot air drying, microwave drying, and freeze drying, on the nutritional and microbial profiles of dried mushrooms. Each method is evaluated for its effectiveness in preserving essential nutrients and reducing microbial load. Current research indicates that freeze drying is particularly effective in preserving nutritional quality, while hot air and microwave drying significantly reduce microbial load. However, more well-designed studies are needed to fully understand the implications of these methods for safety and nutritional benefits. These findings are valuable for optimizing dehydration methods for high-quality dried mushrooms that are suited for culinary and medicinal use.
Open Access: Yes
Bioactive Potential of Actinobacteria Strains Isolated from the Rhizosphere of Lavender, Lemon Balm, and Oregano
Publication Name: Agriculture Switzerland
Publication Date: 2024-10-01
Volume: 14
Issue: 10
Page Range: Unknown
Description:
The objective of this study was to isolate and characterize actinobacteria from the rhizosphere of medicinal and aromatic plants, specifically lavender (Lavandula angustifolia Mill.), lemon balm (Melissa officinalis L.), and oregano (Origanum vulgare L.). Rhizospheric soil samples revealed a high abundance of culturable actinobacteria (6.97–7.23 log10 CFU/g). Six isolates were selected for their promising enzymatic activities (lignin peroxidase, carboxymethyl cellulase) and antimicrobial properties. Isolates M345 and M162 exhibited the highest cellulase activity indices (3.19 ± 0.71 and 2.54 ± 0.22, respectively), with five isolates producing lignin peroxidase. These actinobacteria also demonstrated plant growth-promoting traits such as phosphate solubilization and nitrogen fixation, along with strong antimicrobial activity against Gram-negative bacteria and phytopathogenic fungi. Additionally, they significantly enhanced maize seed germination, increasing the vigor index from 4283.33 ± 1264.37 to 6248.28 ± 1661.94 compared to that of the control. These results indicate that the isolated actinobacteria strains hold potential as microbial inoculants for sustainable agriculture, contributing to soil health, plant growth, and pathogen management.
Open Access: Yes
Evaluation of the physical, antioxidant, and organoleptic properties of biscuits fortified with edible flower powders
Publication Name: Food Science and Nutrition
Publication Date: 2024-05-01
Volume: 12
Issue: 5
Page Range: 3265-3272
Description:
Foods enriched with plants have gradually become an area of increasing research interest because plant ingredients may offer several positive effects on human health and the body. The aim of our study was to develop biscuits fortified with three different types of edible flowers (marigold, lavender, and rose) and examine their physical and antioxidant characteristics, as well as consumer acceptability. The antioxidant properties and characterization of biscuits highlighted that edible flowers may cause increased total polyphenol and total flavonoid yields, as well as DPPH radical scavenging activities. Concerning biscuits fortified with rose petals, the total monomer anthocyanin content was also raised. In addition, the results showed that the antioxidant properties of biscuits increased with increasing concentration (from 2.5% to 5.0%) of edible flowers. Despite this, the consumer acceptability results clearly showed that the addition of marigold and lavender at a concentration of 5.0% caused significantly decreased overall acceptance. We found that the fortification step may increase the spread ratio, which is an important quality attribute of biscuits. We found slight variations in the diameter, thickness, and baking loss parameters of fortified biscuits compared to the control. All in all, the best results were obtained when the biscuits were fortified with rose petals at a concentration of 5%.
Open Access: Yes
DOI: 10.1002/fsn3.3993
Wild Blackberry Fruit (Rubus fruticosus L.) as Potential Functional Ingredient in Food: Ultrasound-Assisted Extraction Optimization, Ripening Period Evaluation, Application in Muffin, and Consumer Acceptance
Publication Name: Foods
Publication Date: 2024-03-01
Volume: 13
Issue: 5
Page Range: Unknown
Description:
The aim of the present study is to evaluate the antioxidant properties of wild blackberry fruits as well as their possible use in powdered form as a functional ingredient. For this, ultrasound-assisted extraction optimization, ripening stage evaluation, and wild blackberry powder incorporation into a real food matrix were applied. The optimum conditions for extraction were as follows: 60% MeOH, 20 min of extraction time, acidification with 0.5% HCl, and a 1:40 g/mL solid-to-solvent ratio, which allowed the following yields: total polyphenol content (TPC): 53.8 mg GAE/g; total flavonoid content (TFC): 5.78 mg QE/g; total monomer anthocyanin content (TMA): 11.2 mg CGE/g; 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH): 71.5 mg AAE/g; IC50 : 52.3 µg/mL. The study also highlighted that, during the ripening process, the TPC (41.4%), TFC (17.0%), and DPPH levels (66.4%) of the fruits decreased while the TMA yield increased. The incorporation of blackberry powder at different levels (5–20%) increased the TPC, TFC, TMA, and antioxidant properties of muffins. Although the muffins enriched with 20% wild blackberry powder had the best chemical properties (TPC: 3.15 mg GAE/g; TFC: 0.52 mg QE/g; TMA: 0.23 mg CGE/g; DPPH: 1.70 mg AAE/g; IC50 : 1.65 mg/mL), the sensory analysis showed that the addition of blackberry fruit at a concentration of 10% to the muffins resulted in the best consumer acceptability.
Open Access: Yes
Determination of the health-protective effect of ancient cereals and one possibility of increasing their functionality
Publication Name: Cereal Research Communications
Publication Date: 2024-03-01
Volume: 52
Issue: 1
Page Range: 247-253
Description:
This study aimed to examine the differences between the antioxidant capacity, and the total polyphenol content of the ancient grains namely Einkorn (Triticum monococcum L.) and Khorasan (Triticum turanicum) compared with wheat (Triticum aestivum L.), furthermore attempt to create a functional loaf enriched with rosemary (Salvia Rosmarinus Spenn.). To achieve this goal, 1, 3, and 5% of rosemary were added to the loafs made of Einkorn, Khorasan, and common wheat flour, and then the changes in their antioxidant and polyphenol content were examined. Based on the spectrophotometric results, it can be said that the type of wheat clearly affects the amount of the tested components. In all cases, the loafs made from common wheat contained the least amount of antioxidants (0.74 mg AAE/g) and polyphenols (0.41 mg GAE/g). On the other hand, Khorasan loaves showed outstanding values in terms of their antioxidant (0.89 mg AAE/g) and polyphenol (0.68 mg GAE/g) content. Einkorn loaves contained smaller amounts of the tested antioxidants (0.71 mg AAE/g) and polyphenols (0.51 mg GAE/g), but they were still better than common wheat loaves. The addition of rosemary can significantly increase the amount of compounds with an antioxidant effect in bakery products, but care must be taken to preserve the favorable organoleptic properties. It has been proven that ancient grains have a more positive physiological effect than common wheat, and the health-protective properties of bakery products can be increased by adding herbs. Graphical abstract: (Figure presented.)
Open Access: Yes
Determination of the health-protective effect of different Sempervivum and Jovibarba species
Publication Name: Journal of King Saud University Science
Publication Date: 2024-01-01
Volume: 36
Issue: 1
Page Range: Unknown
Description:
This study aimed to examine the micro- and macroelements, as well as the total antioxidant, and polyphenol content of 22 different types of dried houseleek, and fresh plant decoctions. The measurement of antioxidant and polyphenol content was carried out with two drying methods- a drying cabinet and lyophilization. The plants, without exception, were provided by Berger Trio Kft. from Jánossomorja (Hungary), thus, the influencing effect of the environment is negligible in the case of the tested species. Based on the results, the type of houseleek has a clear influencing effect on the amount of the tested constituents. The investigated microelements were B, Cu, Fe, Mn, and Zn, while Ca, K, Mg, Na, P, and S were examined among the macroelements. In terms of microelement content, Mn was present in outstanding amounts (20.67–148.00 mg/kg DW), while among macroelements Ca (44.43–95.27 g/kg DW) and Na (24.07–128.50 g/kg DW) was present in larger quantities in the samples. It can be said that in the case of samples with a high amount of antioxidants and polyphenols, the element content is low and the reverse is also true. During the examination of the dried houseleeks, outstanding values were obtained for the tested compounds. The antioxidant values of the dried leaves were between 2.22 and 317.74 mg AAE/g DW, while their polyphenol contents ranged from 5.52 to 144.16 mg GAE/g DW, however, the drying methods had some influencing effect. Regarding fresh plant decoctions, the polyphenol contents (0,02–1,11 mg GAE/g FW) were negligible, while the amount of antioxidants (2,38–4,61 mg AAe/g FW) were low. With other solvents (e.g. alcoholic extraction - tinctures), better results are likely to be achieved. Houseleek species (especially in dried form) are an excellent source of trace elements, antioxidants, and phenolic components so they can even be used as additives to functional foods or consumed on their own in the form of encapsulated dietary supplements.
Open Access: Yes
Optimization and validation of HPLC–DAD method for simultaneous analysis of sweeteners, preservatives, and caffeine in sugar-free beverages
Publication Name: European Food Research and Technology
Publication Date: 2023-11-01
Volume: 249
Issue: 11
Page Range: 2797-2805
Description:
In this study, the optimization and performance evaluation of a high-performance liquid chromatography with diode array detection (HPLC–DAD) method for the simultaneous determination of four sweeteners (acesulfame-potassium, saccharin, aspartame, and rebaudioside A), two preservatives (sodium benzoate and potassium sorbate), and caffeine in sugar-free drinks are presented. The separation was carried out using a gradient elution of acetonitrile and phosphate buffer (12.5 mM, pH = 3.3) on a reversed-phase column. Under the optimized conditions, the method allowed for the specific and selective simultaneous separation of all target analytes in less than 9 min. The developed HPLC–DAD method was validated and demonstrated excellent linearity (all analytical curves showed R2 ≥ 0.9995), satisfactory accuracy (recovery values ranging between 94.1 and 99.2% in real samples), and repeatability (intra- and inter-day relative standard deviations were ≤ 2.49%). Overall, 69 products available in the Hungarian market were successfully tested with the applied method.
Open Access: Yes
Antioxidant and polyphenol content of different milk and dairy products
Publication Name: Journal of King Saud University Science
Publication Date: 2023-10-01
Volume: 35
Issue: 7
Page Range: Unknown
Description:
The aim of the present study was to determine the antioxidant capacity and total polyphenol content of raw milk, dairy products (ricotta and cottage cheese), and by-products (sweet and acid whey) from different animal breeds (cow, goat). Overall, the total polyphenol content of raw milk ranged from 420.34 to 490.72 mg GAE/100 mL, while the total antioxidant content changed between 8.95 and 28.72 mg AAE/100 mL. These values in the case of cottage cheeses were 32.29–124.29 mg GAE/100 mL for polyphenols and 14.12–16.38 mg AAE/100 g for antioxidants. Significant differences were observed between the total polyphenol content and antioxidant properties of sweet- (10.85–197.55 mg AAE/100 g antioxidant; 32.29–124.29 mg GAE/100 g polyphenol) and acid whey (13.28–158.69 mg AAE/100 g antioxidant; 43.50–98.03 mg GAE/100 g polyphenol). In addition, slight differences in total polyphenol content (10.55–19.01 mg GAE/100 g) and antioxidant capacity (10.84–15.93 mg AAE/100 g) were observed for ricotta cheeses made from milk of different animal breeds. The results show that milk and dairy products are excellent sources of antioxidants and polyphenols.
Open Access: Yes
Increasing the functionality of sponge cakes by mint, and cocoa powder addition
Publication Name: Heliyon
Publication Date: 2023-09-01
Volume: 9
Issue: 9
Page Range: Unknown
Description:
While sponge cake is one of the most well-liked cookies in the world, mint and cocoa have both been shown to be excellent sources of antioxidant compounds. Therefore, the aim of the study was to create functional sponge cakes with the addition of Dutch cocoa powder and different types of mint, with proven increased total antioxidant and polyphenol content. Additionally, made an effort to produce functional sponge cakes enhanced with cocoa powder and dried, ground mint leaves. To accomplish this, the cakes with cocoa addition were also added 1, 3, and 5% of the ground mint variety, and then the changes in their antioxidant and polyphenol content were assessed. To prove the functionality of cakes, total polyphenol content was detected by Folin-Ciocalteu, while all antioxidant content was measured by the FRAP method. The spectrophotometric analysis supported the functionality of sponge cakes and the baking losses of identified components. The total polyphenol content of baked goods ranged from 1.37 to 1.66 mg GAE/g for peppermint cakes, from 1.66 to 1.87 mg GAE/g for spearmint cakes, and from 1.20 to 1.68 mg GAE/g for strawberry mint sponge cakes. The total antioxidant content of the functional cakes changed between 1.84 and 2.82 mg AAE/g for peppermint cakes, from 1.84 to 4.00 mg AAE/g for spearmint cakes, and from 1.56 to 2.94 mg GAE/g for strawberry mint sponge cakes. The natural control samples, and control sponge cakes made without mint addition with only cocoa powder always had lower levels of polyphenols and antioxidants. All samples had baking loss (control samples had the highest in all cases), but strawberry mint samples had the least of it when it came to antioxidant content and spearmint samples had the least in the case of polyphenol content. Overall, mints and Dutch cocoa powder are appropriate for the production of functional bakery goods because they give the final product a tasty flavor and provide a significant amount of antioxidants and polyphenols despite baking.
Open Access: Yes
Effect of Simulated Transport Conditions on Microbiological Properties of Bottled Natural Mineral Water
Publication Name: Water Switzerland
Publication Date: 2023-05-01
Volume: 15
Issue: 9
Page Range: Unknown
Description:
Bottled mineral water is distributed globally through complex supply chains, making it available far beyond its bottling plants. In low-viscosity food matrices, invisible changes may occur due to shaking. The primary purpose of this research was to investigate the potential correlation between the intensity of mechanical agitation and the number of detectable microorganisms in bottled mineral water. The simulation of dynamic mechanical vibration was conducted using both time-accelerated and real-time tests. Freshly bottled natural mineral water and commercially available mineral water brands from different bottling locations and times were subjected to random vibration at three intensities as specified by the ASTM D-4169-16 standard, which simulates road transport on semi-trailer trucks. The study investigated the specific growth rate, the generation time, and the maximum cell numbers of microorganisms. The quantitative PCR (qPCR) technique was used to determine and compare the concentrations of microbes. Dynamic mechanical vibration affected the microbiome of mineral waters, influencing growth rates and generation times. In the case of waters from different bottling locations and times, the specific growth rate varied significantly for each water and for each intensity. This finding demonstrates that the microbiome composition of the water source and the interaction between microbes influence the response to mechanical impact. The time-accelerated test was shown to be suitable for analyzing the reaction of the microbiome of the tested matrix to the intensity and duration of vibration. The applied test protocol enabled the monitoring of changes in cell numbers by qPCR. All three intensities of the time-accelerated method were effective in testing the effects of real-time mechanical agitation on the microbiome.
Open Access: Yes
DOI: 10.3390/w15091757
Biocontrol Activity of Aromatic and Medicinal Plants and Their Bioactive Components against Soil-Borne Pathogens
Publication Name: Plants
Publication Date: 2023-02-01
Volume: 12
Issue: 4
Page Range: Unknown
Description:
Soil-borne phytopathogens can have detrimental effects on both cereal and horticultural crops resulting in serious losses worldwide. Due to their high efficiency and easy applicability, synthetic pesticides are still the primary choice in modern plant disease control systems, but stringent regulations and increasing environmental concerns make the search for sustainable alternatives more pressing than ever. In addition to the incorporation of botanicals into agricultural practices, the diversification of cropping systems with aromatic and medicinal plants is also an effective tool to control plant diseases through providing nutrients and shaping soil microbial communities. However, these techniques are not universally accepted and may negatively affect soil fertility if their application is not thoroughly controlled. Because the biocontrol potential of aromatic and medicinal plants has been extensively examined over the past decades, the present study aims to overview the recent literature concerning the biopesticide effect of secondary metabolites derived from aromatic and medicinal plants on important soil-borne plant pathogens including bacteria, fungi, and nematodes. Most of the investigated herbs belong to the family of Lamiaceae (e.g., Origanum spp., Salvia spp., Thymus spp., Mentha spp., etc.) and have been associated with potent antimicrobial activity, primarily due to their chemical constituents. The most frequently tested organisms include fungi, such as Rhizoctonia spp., Fusarium spp., and Phytophthora spp., which may be highly persistent in soil. Despite the intense research efforts dedicated to the development of plant-based pesticides, only a few species of aromatic herbs are utilized for the production of commercial formulations due to inconsistent efficiency, lack of field verification, costs, and prolonged authorization requirements. However, recycling the wastes from aromatic and medicinal plant-utilizing industries may offer an economically feasible way to improve soil health and reduce environmental burdens at the same time. Overall, this review provides comprehensive knowledge on the efficiency of aromatic herb-based plant protection techniques, and it also highlights the importance of exploiting the residues generated by aromatic plant-utilizing sectors as part of agro-industrial processes.
Open Access: Yes
Analysis of Caffeine and Antioxidant Content of Ethiopian Coffee Varieties from Different Growing Areas
Publication Name: Journal of Food Processing and Preservation
Publication Date: 2023-01-01
Volume: 2023
Issue: Unknown
Page Range: Unknown
Description:
Quality coffee consumption is increasing nowadays; however, quality can only be achieved through appropriate cultivation and processing techniques. The study is aimed at using high-performance liquid chromatography (HPLC) and spectrophotometric techniques for evaluating different coffee varieties grown in Ethiopia based on caffeine, antioxidant, and polyphenol content. Despite coming from various coffee-growing regions, all of the tested coffee varieties (heirloom, forest coffee, heirloom 2, bourbon, mixed heirloom, and catuai) underwent wet processing. The data revealed that the caffeine content ranged from 0.14 to 0.37 mg/g, total antioxidant content from 63.75 to 78.05 mg AAE/g, and total polyphenol content from 27.08 to 31.04 mg GAE/g in the selected varieties. The findings show that the amount of the identified compounds is significantly (p ≤ 0 05) influenced by the variety type, growing area, and processing (intensity of roasting). The different coffee varieties were also tested for sensory quality, and the findings suggest that the typical coffee drinker prefers fruity flavors in their coffee, along with medium acidity and roasting intensity levels. The cultivation and processing technologies used in coffee production have a significant impact on the quality and sensory enjoyment of coffee.
Open Access: Yes
DOI: 10.1155/2023/8831024
Composting of medicinal and aromatic plant waste: challenges and opportunities
Publication Name: Recent Trends in Solid Waste Management
Publication Date: 2023-01-01
Volume: Unknown
Issue: Unknown
Page Range: 115-136
Description:
Aromatic plants have been extensively used for their medicinal and culinary properties by the cosmetic, pharmaceutical, and food industries. Along with the prosperous development of the herbal industry, however, enormous amounts of solid biomass have been generated, creating an environmental hazard. Since the waste material generated during harvesting, pre-processing, or extraction may retain its nutritional value, it can be converted to compost or vermicompost. Nonetheless, the addition of herbal residues to the feedstock material may negatively influence biodegradation due to the lower nitrogen content, increased lignocellulose content, and the remaining bioactive compounds. Overall, this book chapter discusses the current challenges that herbal waste composting/vermicomposting faces, provides insights into the process optimisation, and summarises the potential beneficial effects of mature compost.
Open Access: Yes
The effects of extraction conditions on the antioxidant activities, total polyphenol and monomer anthocyanin contents of six edible fruits growing wild in Hungary
Publication Name: Heliyon
Publication Date: 2022-12-01
Volume: 8
Issue: 12
Page Range: Unknown
Description:
Although wild fruits are significantly underutilized in most countries, they could be good sources of valuable bioactive compounds with antioxidant properties. Therefore the present study focused on the study of a conventional extraction technique (maceration with shaking; MACS ) to extract natural antioxidants and anthocyanin colorants from six edible wild-growing fruits (European crab apple, bilberry, yellow-, red-, and purple-skinned greengage, and quince). One-factor-at-a-time (OFAT) methodology was chosen to investigate the influences of three different parameters (solvent type, extraction time and solvent acidity) on the total polyphenol contents (TPCs), total monomeric anthocyanin (TMA) contents, and antioxidant capacities, specifically ferric reducing power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity (DPPH). After optimization, the recorded TPCs and antioxidant activities proved to be significantly higher for all analyzed fruits when compared to differing extraction conditions. For European crab apple and purple-skinned greengage, the best extraction conditions were a ratio of 80:20 (v/v) EtOH–H2 O, 1% (v/v) of HCOOH, and an extraction time of 90 min. In the case of red-skinned greengage, the extraction parameters were the same as the above except for the acid concentration (0.5%; v/v) used. For quince, the optimized conditions required a 50:50 (v/v) EtOH–H2 O mixture, an extraction time of 90 min, and 0.5% (v/v) HCOOH concentration. The best conditions for the extraction of bilberry and yellow-skinned greengage were an EtOH–H2 O combination of 50:50 (v/v), extraction time of 60 min, and HCOOH concentration of 0.5% (v/v). The highest TPC and antioxidant activity were observed in quince (281–510 mg GAE/100g and 109–395 mg AAE/100g) whereas the lowest were measured in European crab apple (55.9–70.0 mg GAE/100g and 20.1–43.2 mg AAE/100g). Bilberry exhibited the highest TMA content (346 mg CGE/100g). Overall, our results showed that these wild fruits could be a good source of natural antioxidants for the local residents.
Open Access: Yes
Effect of Thymus vulgaris L. essential oil and thymol on the microbiological properties of meat and meat products: A review
Publication Name: Heliyon
Publication Date: 2022-10-01
Volume: 8
Issue: 10
Page Range: Unknown
Description:
Since foodborne diseases are often considered as one of the biggest public health threats worldwide, effective preservation strategies are needed to inhibit the growth of undesirable microorganisms in food commodities. Up to now, several techniques have been adopted for the production of safe and high-quality products. Although the traditional methods can improve the reliability, safety, and shelf-life of food, some of them cannot be applied without rising health concerns. Thereby, the addition of various phytochemicals has gained much attention during the last decades, especially for meat products that may be contaminated with pathogenic and spoilage organisms. Thyme (Thymus vulgaris L.), as an important medicinal and culinary herb, is a promising source of bioactive compounds that have a great impact on the microbiological stability of meat by suppressing the undesirable microflora. However, the use of these antimicrobials is still facing difficulties due to their aromatic properties and variable efficacy against targeted species. In this paper, we provide an overview on the potential effects of thyme essential oil (EO) and thymol as bio-preservative agents in meat products. Furthermore, this paper provides insights into the limitations and current challenges of the addition of EOs and their constituents to meat commodities and suggests viable solutions that can improve the applicability of these phytochemicals.
Open Access: Yes
The beneficial effect of peppermint (Mentha X Piperita L.) and lemongrass (Melissa officinalis L.) dosage on total antioxidant and polyphenol content during alcoholic fermentation
Publication Name: Food Chemistry X
Publication Date: 2022-03-30
Volume: 13
Issue: Unknown
Page Range: Unknown
Description:
Our research aimed to create an herbal fermented alcoholic beverage with high antioxidant and polyphenol content. In this study, continuous sampling was performed throughout the fermentation period, and the changes in total antioxidant (TA) and total polyphenol (TP) contents were determined. After processing the raw material, the prepared herbs were added in 0.5 and 1.0 v/v% concentrations to the samples. The TP content of the control sample was between 1.17 and 1.57 mg/g, and the TA content was 2.12 and 2.54 mg/g during the fermentation process. The lemongrass dosage increased 77.86 % the antioxidant and 70.98 % the polyphenol content by the end of the fermentation process. In the best case, the peppermint dosage increased 72.80 % of the antioxidant content and 72.05 % of the polyphenol content. Overall, fermentation combined with herbs dosage could increase the bioavailability of products made from its polyphenol and antioxidant contents and can be used to develop novel functional foods.
Open Access: Yes
Analytical procedures for determination of phenolics active herbal ingredients in fortified functional foods: an overview
Publication Name: European Food Research and Technology
Publication Date: 2022-02-01
Volume: 248
Issue: 2
Page Range: 329-344
Description:
Fortification of foods with phenolic compounds is becoming increasingly popular due to their beneficial physiological effects. The biological activities reported include antioxidant, anticancer, antidiabetic, anti-inflammatory, or neuroprotective effects. However, the analysis of polyphenols in functional food matrices is a difficult task because of the complexity of the matrix. The main challenge is that polyphenols can interact with other food components, such as carbohydrates, proteins, or lipids. The chemical reactions that occur during the baking technologies in the bakery and biscuit industry may also affect the results of measurements. The analysis of polyphenols found in fortified foods can be done by several techniques, such as liquid chromatography (HPLC and UPLC), gas chromatography (GC), or spectrophotometry (TPC, DPPH, FRAP assay etc.). This paper aims to review the available information on analytical methods to fortified foodstuffs while as presenting the advantages and limitations of each technique.
Open Access: Yes
Influence of microbial inoculants on co-composting of lignocellulosic crop residues with farm animal manure: A review
Publication Name: Journal of Environmental Management
Publication Date: 2022-01-15
Volume: 302
Issue: Unknown
Page Range: Unknown
Description:
The rapidly developing agro-industry generates huge amounts of lignocellulosic crop residues and animal manure worldwide. Although co-composting represents a promising and cost-effective method to treat various agricultural wastes simultaneously, poor composting efficiency prolongs total completion time and deteriorates the quality of the final product. However, supplementation of the feedstock with beneficial microorganisms can mitigate these negative effects by facilitating the decomposition of recalcitrant materials, enhancing microbial enzyme activity, and promoting maturation and humus formation during the composting process. Nevertheless, the influence of microbial inoculation may vary greatly depending on certain factors, such as start-up parameters, structure of the feedstock, time of inoculation, and composition of the microbial cultures used. The purpose of this contribution is to review recent developments in co-composting procedures involving different lignocellulosic crop residues and farm animal manure combined with microbial inoculation strategies. To evaluate the effectiveness of microbial additives, the results reported in a large number of peer-reviewed articles were compared in terms of composting process parameters (i.e., temperature, microbial activity, total organic carbon and nitrogen contents, decomposition rate of lignocellulose fractions, etc.) and compost characteristics (humification, C/N ratio, macronutrient content, and germination index). Most studies confirmed that the use of microbial amendments in the co-composting process is an efficient way to facilitate biodegradation and improve the sustainable management of agricultural wastes. Overall, this review paper provides insights into various inoculation techniques, identifies the limitations and current challenges of co-composting, especially with microbial inoculation, and recommends areas for further research in this field.
Open Access: Yes
Development of New HS–SPME–GC–MS Technique to the Measurement of Volatile Terpenoid Profile of Milk
Publication Name: Food Analytical Methods
Publication Date: 2021-12-01
Volume: 14
Issue: 12
Page Range: 2585-2596
Description:
This study presented the development of such a HS–SPME–GC–MS technique, with the use of which, directly from the raw milk sample matrix, both qualitatively and quantitatively; the volatile terpenoids (α-pinene, sabinene, β-pinene, p-cymene, limonene, linalool, α-thujene, camphor, menthol, methyl chavicol, caryophyllene E, α-humulene) can be determined, derived from herbs eaten by the dairy animals by different feeding methods. Repeatability was less than 10% in the case of milk fat samples. The estimated limits of quantitation were between 2 and 16 ng/g. The lowest values were 2 ng/g for p-cymene and methyl chavicol; the highest value was 16 ng/g for caryophyllene. In the case of goat milk, the repeatability was better than 8% except for α-thujene. The estimated limits of quantitation were between 1 and 8 ng/g. The lowest values were 1 ng/g for β-pinene, p-cymene and limonene, and the highest value was 8 ng/g for linalool. In milk fat, the highest concentration was identified in caryophyllene E (470 ng/g) and α-humulene (430 ng/g), while the lowest concentration was in p-cymene (2 ng/g) and camphor (2 ng/g). In goat milk, limonene was present in all samples, but its amount varied depending on the type of consumed herb. Methyl chavicol and caryophyllene E were detected in goat’s milk only in one case. The former was detected in sage milk at 2.09 ng/g and the latter in tarragon milk at 2.28 ng/g. We have also successfully demonstrated that the feed consumed by dairy animals also affects the quality of dairy products.
Open Access: Yes
Antioxidant and polyphenol content of different Vitis vinifera seed cultivars and two facilities of production of a functional bakery product
Publication Name: Chemical Papers
Publication Date: 2021-11-01
Volume: 75
Issue: 11
Page Range: 5711-5717
Description:
This study aimed to examine the differences between the antioxidant capacity, and the total polyphenol content of the seed of eight different grape varieties. We also attempt to create a functional loaf enriched with grape seed meal. To achieve this goal, 3, 6, and 9% of the grape seed meal were added to the loaves made of bread flour and gluten-free flour mixture, and then the changes in their antioxidant and polyphenol content were examined. Based on the results, there were significant differences between the polyphenol and antioxidant content of grape seed varieties. The antioxidant content of the grape seeds varied between 228.50 mg AAE/g (94.80 mg TE/g) and 438.33 mg AAE/g (181.86 mg TE/g) in case of fatty samples, and between 176.29 mg AAE/g (41.24 mg TE/g) and 424.91 mg AAE/g (99.40 mg TE/g) in case of defatted samples. The total polyphenol content of the fatty samples changed between 91.16 and 221.81 mg GAE/g; in case of defatted samples, it ranged from 46.01 to 207.68 mg GAE/g. Rhine Riesling contained the greatest amount of these compounds. The functionality of loafs was confirmed by spectrophotometric analysis. Total polyphenol content ranged from 0.91 to 3.16 mg GAE/g in case of wheat loafs, and from 1.39 to 5.92 mg GAE/g in case of gluten-free loafs, while the total antioxidant content changed between 0.70 and 6.44 mg AAE/g in case of wheat loafs, and ranged from 2.55 to 9.75 in case of gluten-free loafs. Graphic abstract: [Figure not available: see fulltext.]
Open Access: Yes
Investigation of the long-term stability of various tinctures belonging to the lamiaceae family by HPLC and spectrophotometry method
Publication Name: Chemical Papers
Publication Date: 2021-11-01
Volume: 75
Issue: 11
Page Range: 5781-5791
Description:
The aim of the current study was to analyze the stability of rosmarinic acid in ethanolic tinctures of lemon balm (Melissa officinalis L.), oregano (Origanum vulgare L.), peppermint (Mentha x piperita), rosemary (Rosmarinus officinalis L.), sage (Salvia officinalis L.), and thyme (Thymus vulgaris L.). High-performance liquid chromatography with diode-array detection (HPLC–DAD) was employed to monitor the concentration of the marker compound over a six month period. Furthermore, the tinctures were also evaluated for caffeic acid, total phenolic content, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. We observed that the concentration of rosmarinic acid in tincture stored in closed amber glasses at ambient temperature decreased significantly during 6-month storage. Furthermore, our study squarely confirms the fact that a part of rosmarinic acid is converted to caffeic acid. The tested tinctures can be listed in the following order according to the greatest stability of the marker compound: rosemary > peppermint > oregano > lemon balm > thyme > sage. The results of the study indicated a linear relationship between DPPH values and total phenolic (R2 = 0.92) or rosmarinic acid (R2 = 0.85) contents.
Open Access: Yes
Exploring the rosmarinic acid profile of dark chocolate fortified with freeze-dried lemon balm extract using conventional and non-conventional extraction techniques
Publication Name: Lwt
Publication Date: 2021-07-01
Volume: 147
Issue: Unknown
Page Range: Unknown
Description:
The present study evaluated the feasibility of the fortification of dark chocolate with a freeze-dried lemon balm extract rich in rosmarinic acid (RA). Given the food matrix used, it was necessary to develop an analytical and extraction method for RA determination in fortified chocolate. A simple and efficient reversed-phase high-performance liquid chromatography method (RP-HPLC) has been developed for the estimation of RA in dark chocolate. The validity of the method was demonstrated by evaluating the system suitability, linearity, precision, accuracy, the limit of detection, and limit of quantification. The extraction of RA from chocolate was optimized using maceration with shaking (MACS ) and microwave-assisted extraction (MAE). The experimental conditions, namely solvent type and extraction time, were optimized using the one-factor-at-a-time methodology. Under the optimized conditions, the proposed method showed good recovery within 95% and acceptable reproducibility with intra- and inter-day relative standard deviations of <2.3%. The method was linear from 5 to 800 μg/mL with a determination coefficient of 1.000. Moreover, the functional chocolate showed a homogeneous active ingredient distribution and retained its RA amount since no significant difference was observed during storage. Overall, the present method was successfully applied to determine RA content in fortified chocolate.
Open Access: Yes
Study of the antifungal effect of a copper-containing foliar fertilizer
Publication Name: Cereal Research Communications
Publication Date: 2021-06-01
Volume: 49
Issue: 2
Page Range: 337-341
Description:
Winter wheat (Triticum aestivum L.) is one of the most important arable crops. Copper-containing fertilizers are used in crop production to treat or reduce the negative effects of copper deficiency. Various copper compounds are also widely used as effective fungicide agents. The influence of a copper-containing foliar fertilizer prepared from a copper solution and sucrose had been tested on the yield and raw protein content of winter wheat in previous field experiments. In this work, we studied the antifungal effect of this particular foliar fertilizer in an in vitro trial, wherein the fungicide effect on growth and reproduction of specific plant pathogens, i.e., Fusarium graminearum and Drechslera sorokiniana, was evaluated with the agar well diffusion method. The results showed that doses of the foliar fertilizer corresponding to field applications of at least 1 kg ha−1 had antifungal effects, which were significant (P < 0.01) above the concentration level of 3337 mg L−1, corresponding to 2 kg ha−1 application, against both fungal pathogens. A linear correlation (r2 > 0.95) was found between copper concentration and fungicide efficiency in the concentration range studied. The fungicide efficiency of the foliar fertilizer was lower than that of the commercially available copper-oxychloride fungicide. The sole use of the foliar nutrient tested probably cannot replace fungicide treatments. However, in addition to its plant nutrition effect, its antifungal properties should also be taken into consideration.
Open Access: Yes
Effect of bacterial inoculation on co-composting of lavender (Lavandula angustifolia Mill.) waste and cattle manure
Publication Name: 3 Biotech
Publication Date: 2021-06-01
Volume: 11
Issue: 6
Page Range: Unknown
Description:
The primary purpose of this study was to investigate the influence of Cellulomonas flavigena and Streptomyces viridosporus, as a bacterial inoculant, on the compostability of post-extraction lavender waste. The major physicochemical, microbiological, and biological properties of the composting materials were monitored for 161 days. The technology developed was shown to improve the compostability of recalcitrant herbal residues. The use of lavender waste beneficially affected the composting process by extending the thermophilic phase, accelerating the degradation of organic matter, and elevating the viable counts of useful microorganisms; however, adverse effects were also observed, including an increased carbon-to-nitrogen ratio (19.05) and a decreased germination index (93.4%). Bacterial inoculation was found to preserve the nitrogen content (2.50%) and improve the efficiency of biodegradation. The Salmonella- and Escherichia coli-free final composting products were mature, stable, and ready for soil application. To the authors’ knowledge, no previous research has investigated the compostability of lavender waste. Likewise, this is the first study that has used strains of C. flavigena and S. viridosporus in combination to facilitate a composting process.
Open Access: Yes
Review on the occurrence of the mcr-1 gene causing colistin resistance in cow's milk and dairy products
Publication Name: Heliyon
Publication Date: 2021-04-01
Volume: 7
Issue: 4
Page Range: Unknown
Description:
Both livestock farmers and the clinic use significant amount of antibiotics worldwide, in many cases the same kind. Antibiotic resistance is not a new phenomenon, however, it is a matter of concern that resistance genes (mcr - Mobilized Colistin Resistance - genes) that render last-resort drugs (Colistin) ineffective, have already evolved. Nowadays, there is a significant consumption of milk and dairy products, which, if not treated properly, can contain bacteria (mainly Gram-negative bacteria). We collected articles and reviews in which Gram-negative bacteria carrying the mcr-1 gene have been detected in milk, dairy products, or cattle. Reports have shown that although the incidence is still low, unfortunately the gene has been detected in some dairy products on almost every continent. In the interest of our health, the use of colistin in livestock farming must be banned as soon as possible, and new treatments should be applied so that we can continue to have a chance in fighting multidrug-resistant bacteria in human medicine.
Open Access: Yes
Linseed oil supplementation affects fatty acid desaturase 2, peroxisome proliferator activated receptor gamma, and insulin-like growth factor 1 gene expression in turkeys (Meleagris gallopavo)
Publication Name: Animal Bioscience
Publication Date: 2021-04-01
Volume: 34
Issue: 4
Page Range: 662-669
Description:
Objective: Effects of linseed oil (LO) supplementation on the fat content and fatty acid profile of breast meat, and the expression of three genes in the liver, breast muscle and fat tissues of commercial 154-day-old hybrid male turkeys were investigated. Methods: The animals in the control group were fed a commercially available feed and received no LO supplementation (n = 70), whereas animals in the LO group (n = 70) were fed the same basic diet supplemented with LO (day 15 to 21, 0.5%; day 22 to 112, 1%). The effect of dietary LO supplementation on fatty acid composition of breast muscle was examined by gas chromatography, and the expression of fatty acid desaturase 2 (FADS2), peroxisome proliferator activated receptor gamma (PPARγ), and insulin-like growth factor 1 (IGF1) genes was analysed by means of quantitative reverse transcription polymerase chain reaction. Results: The LO supplementation affected the fatty acid composition of breast muscle. Hepatic FADS2 levels were considerably lower (p<0.001), while adipose tissue expression was higher (p<0.05) in the control compared to the LO group. The PPARγ expression was lower (p<0.05), whereas IGF1 was higher (p<0.05) in the fat of control animals. There were no significant (p>0.05) differences in FADS2, PPARγ, and IGF1 gene expressions of breast muscle; however, omega-6/omega-3 ratio of breast muscle substantially decreased (p<0.001) in the LO group compared to control. Conclusion: Fatty acid composition of breast meat was positively influenced by LO supplementation without deterioration of fattening parameters. Remarkably, increased FADS2 expression in the liver of LO supplemented animals was associated with a significantly decreased omega-6/omega-3 ratio, providing a potentially healthier meat product for human consumption. Increased PPARγ expression in fat tissue of the LO group was not associated with fat content of muscle, whereas a decreased IGF1 expression in fat tissue was associated with a trend of decreasing fat content in muscle of the experimental LO group.
Open Access: Yes
DOI: 10.5713/ajas.20.0030
Co-composting with herbal wastes: Potential effects of essential oil residues on microbial pathogens during composting
Publication Name: Critical Reviews in Environmental Science and Technology
Publication Date: 2021-01-01
Volume: 51
Issue: 5
Page Range: 457-511
Description:
Because of the increasing use of herbal essential oils (EO) in pharmacology, medicine, and food processing, global EO production grows at a rapid rate, generating large amounts of plant residues worldwide. The use of herbal residues for co-composting with various wastes offers a novel option for eco-friendly and cost effective waste management. However, the biologically active compounds remaining in the herb residues may affect microbial activity in the composting biomass, thereby influencing final compost quality. In this review, we summarize and evaluate current literature on the occurrence and survival of pathogens in composts, the beneficial effects of using herbal residues as bulking agents, and the potential antipathogenic effects of six herb species belonging to the Lamiaceae family as follows: lemon balm (Melissa officinalis L.), oregano (Origanum vulgare L.), lavender (Lavandula angustifolia Mill.), rosemary (Rosmarinus officinalis L.), thyme (Thymus vulgaris L.), and sage (Salvia officinalis L.).
Open Access: Yes
Characterization of fatty acid, antioxidant, and polyphenol content of grape seed oil from different Vitis vinifera L. varieties
Publication Name: OCL Oilseeds and Fats Crops and Lipids
Publication Date: 2021-01-01
Volume: 28
Issue: Unknown
Page Range: Unknown
Description:
In this study, we examined the yield and oil quality of eight different grape varieties. For the experiments, the grape seeds were obtained from the Benedictine Pannonhalma Archabbey in the northwestern region of Hungary. The aim of the studies was to determine the oil yield obtained by extraction and to examine the differences between the fatty acid composition, antioxidant capacity, and total polyphenol content of the oils of different grape varieties. Based on the results, the oil content of the grape seeds varied between 99.91 g/kg and 126.74 g/kg. The grape seed oils analysed contained significant amounts of stearic acid (3.42-9.93%), palmitic acid (7.81-10.66%), oleic acid (14.29-19.92%), and linoleic acid (66.85-72.47%). Besides, the grape seed oils tested contained several other fatty acids in small amounts. There were significant differences in the total antioxidant and total polyphenol content of the oils. Total polyphenol content ranged from 0.24 to 1.13 mg GAE/g, while the total antioxidant content changed between 0.12 and 0.78 μg TEAC/g. The results show that the studied varieties are suitable for the production of table grape seed oil based on their oil yield, and the oils have favourable, health-protecting properties in terms of their quality.
Open Access: Yes
DOI: 10.1051/ocl/2021017
Investigation of the shelf life of fruit yogurts as a function of the treatment of flavoring substances
Publication Name: Elelmiszervizsgalati Kozlemenyek
Publication Date: 2021-01-01
Volume: 67
Issue: 1
Page Range: 3293-3314
Description:
1. SUMMARY Milk and dairy products represent one of the foundations of the human diet because of their valuable ingredients and pleasant sensory properties. The aim of our research was to investigate how different heat treatment processes (microwave irradiation, drying) affect the shelf life of dairy products (yogurt) from a microbiological point of view. In the course of our measurements, the effects of the different heat treatment parameters of the flavoring substances used in the production of the products (apples, bananas) on the microbiological properties of the products and, thus, on their shelf life were investigated. In our experiments, conventional drying (55 °C, 24 hours) and microwave irradiation technology (800 W, 55 °C, 10 min) were used as treatment forms of the additives. Comparisons were made in terms of microbiological parameters (total viable count, yeast/mold count and E. coli/coliform count). Based on our results, we believe that the drying process can ensure microbiological safety in food production if the air circulating in the equipment has adequate hygienic properties. The microwave irradiation technology can be used successfully to inhibit microbes in foods, in this case fruits. However, the same treatment parameters cannot be applied to different fruits.
Open Access: Yes
DAIRY WASTEWATER UTILIZATION BY COMBINATION OF OXIDATION PRE-TREATMENT AND ULTRAFILTRATION
Publication Name: Environmental Engineering and Management Journal
Publication Date: 2021-01-01
Volume: 20
Issue: 12
Page Range: 1711-1719
Description:
In this work the effects of oxidation pre-treatments (ozonation and Fenton reaction) on membrane filtration of dairy wastewater were investigated, together with the further utilization possibilities of residual wastes. The oxidation pre-treatments enhanced the membrane filtration efficiency, from which the short-term ozone treatment was found to be the most effective method both in terms of increasing the flux and the pollutant removal efficiency. After ozone pre-treatment the phosphate and ammonium content of the wastewater migrated to the permeate, while after Fenton pre-treatment the phosphate content remained in the concentrate, and the ammonium content in the permeate decreased. The concentrate was utilized as biogas resource and it was found that both pretreatments increased the biogas production and its methane content as well. Based on our results it can be concluded that ozone pretreatment combined with membrane separation have improved the efficiency of dairy wastewater treatment and may suit the requirements of circular economy.
Open Access: Yes
DOI: DOI not available
Application of the spme technique for determinate the quality indicators of dairy products
Publication Name: Elelmiszervizsgalati Kozlemenyek
Publication Date: 2020-12-01
Volume: 66
Issue: 4
Page Range: 3188-3201
Description:
Solid Phase Microextraction (SPME) is a relatively new extraction technique that combines taking samples from a mixture with a simpler desorption of the components to be analyzed of the unenriched sample in its original state in the analytical instrument. Based on a large number of publications in the literature, the authors describe the possibilities of using the SPME technique in the case of dairy products. The main groups of components that can be detected by the SPME sampling procedure, such as fatty acids, aldehydes and ketones, esters, alcohols, sulfur-containing organic compounds, furans, phenols and terpenoids are presented, through examples in the literature. Due to the limited scope of the manuscript, the authors report only on the possibilities of sample preparation using the SPME technique. Instrumental analytical methods following solid state microextraction, mainly using gas or liquid chromatography, are not described. Details of instrumental analyses are available in the cited literature sources.
Open Access: Yes
DOI: DOI not available
Conventional and nonconventional extraction techniques for optimal extraction processes of rosmarinic acid from six Lamiaceae plants as determined by HPLC-DAD measurement
Publication Name: Journal of Pharmaceutical and Biomedical Analysis
Publication Date: 2020-05-30
Volume: 184
Issue: Unknown
Page Range: Unknown
Description:
The goal of this study was to improve the extraction efficiency of rosmarinic acid (RA) from Lamiaceae herbs (lemon balm, peppermint, oregano, rosemary, sage, and thyme) using various extraction techniques (maceration with stirring, MACS ; heat reflux, HRE; and microwave-assisted extraction, MAE) and extraction conditions (solvent acidity, solvent type, extraction time and temperature). The RA content was measured by high-performance liquid chromatography with diode-array detection (HPLC-DAD) under test conditions. Our results showed that extraction with acidified aqueous ethanol (EtOH-H2 O-HCl, 70:29:1, v/v/v) was the best choice for the recovery of RA compared to other solvent systems. Further study suggested the following optimal extraction times for the different techniques: 120 min at 25 °C with MACS , 15 min at boiling point with HRE, and 5 min at 50 °C and 80 °C with MAE. Based on our results, we demonstrated that by careful adjustment of the extraction conditions, it is possible to set up a single extraction protocol to extract RA from different plants.
Open Access: Yes
Purification of model dairy wastewaters by Ozone, Fenton pre-treatment and membrane filtration
Publication Name: Periodica Polytechnica Chemical Engineering
Publication Date: 2020-01-01
Volume: 64
Issue: 3
Page Range: 357-363
Description:
This study aimed to investigate the effect of ozone and Fenton-reaction as a pre-treatment before ultrafiltration of model dairy waste waters containing sodium caseinate. Filtration resistances and pollutant retentions were determined and compared. It was found, that both pre-treatment increased the retention, achieving almost 100 % pollutant elimination efficiency after short term pre-oxidation. The effect of Fe-ion concentration on Fenton pretreatment efficiency also was examined, and it was found that higher concentration resulted in decreased filtration resistances, revealing that coagulation-flocculation effect of reactants has dominant role in the protein separation efficiency. The presence of lactose also affects the pollutant removal efficiency, it promotes fouling in presence of Fenton reagents.
Open Access: Yes
DOI: 10.3311/PPch.15046
Recent advances in the analysis of rosmarinic acid from herbs in the lamiaceae family
Publication Name: Natural Product Communications
Publication Date: 2019-01-01
Volume: 14
Issue: 7
Page Range: Unknown
Description:
Rosmarinic acid (RA) is a phenolic acid found in a variety of plants, especially those in the Lamiaceae family. A number of biological effects are attributed to the compound; so, in recent years, RA has been the focus of many studies. The aim of this review is to provide information on the latest developments on flow chromatography analysis of RA. A reverse-phase high-performance liquid chromatography (HPLC) method with gradient elution and either diode array or mass spectrometric detection is usually used to measure the compound. Factors affecting the performance of HPLC analysis of RA such as sample preparation, column, mobile phase, and detection methods are discussed in detail in this review.
Open Access: Yes
Microwave steam explosion and enzymatic hydrolysis of vine-branch
Publication Name: Acta Alimentaria
Publication Date: 2018-09-01
Volume: 47
Issue: 4
Page Range: 443-452
Description:
Our research target was to utilise vine-branch, existing in huge amounts, for energetic purposes. During our experiments, microwave (MW) treatments of different powers (400-1600 W), pressures (1-5 bar), temperatures (120-180 °C), and treatment times (3-30 min) were applied to change the physical condition of vine-branch. After MW, enzymatic hydrolysis (EH) was used (85-100 h, 37 °C). In addition, beside MW, comparisons were made regarding various treatment methods: untreated (UTE), cooking plate (CP), and autoclave (AC), to determine to what extent they affect the final glucose yield. This yield can even further be increased by MW pre-treatment (50 W, 3-30 min, 40 °C) of the enzyme used during the hydrolysis, which reinforces the argument that enzyme activity can be increased by irradiation. A difference of 22.1% was detected among the glucose yield values in untreated and treated enzyme processes.
Open Access: Yes
Evaluation of Antibacterial Properties of Commercial Essential Oils on Foodborne Pathogens in a Liver Pâté-Type Product
Publication Name: Chemical Engineering Transactions
Publication Date: 2023-01-01
Volume: 107
Issue: Unknown
Page Range: 253-258
Description:
In the past, several efforts have been made to find suitable substitutes for synthetic preservatives in meat products with fewer side effects on human health. Essential oils have gained worldwide interest due to their antimicrobial activity. The addition of these aromatic compounds to foods may be hampered by their strong sensory characteristics (taste and smell). The objective of this study was to evaluate the efficiency of five essential oils (EOs) (Ocimum basilicum L., Origanum vulgare L., Rosmarinus officinalis L., Salvia officinalis L., and Thymus vulgaris L.) against important foodborne pathogens. First, a microdilution assay was carried out to determine the minimal inhibitory concentration (MIC) of the EOs against Staphylococcus (Staph.) aureus ATCC 6538, Salmonella (S.) enterica subsp. enterica serovar Typhimurium ATCC 14028, and Escherichia (E.) coli ATCC 25922. Since the EOs inhibited the growth of these bacteria, their activity was studied in a real food matrix. The in vivo test was performed on the model of liver pâté: the homogenized and heat-treated samples were formulated with EOs at concentrations of 1 MIC and 2 MIC, inoculated with bacterial suspensions (105 CFU/g), packaged under vacuum, and stored at 4 ºC for 3 days. The addition of 2 MIC of thyme and sage oils showed a significant reduction in the viable counts of E. coli, S. Typhimurium, and Staph. aureus compared to the control samples. Overall, this study demonstrated that thyme and sage EOs, as natural preservatives, had great potential to prevent the growth of important foodborne pathogens (E. coli, S. Typhimurium, and Staph. aureus) in liver pâté, but their efficiency was highly dose-dependent. However, the tested concentrations of EOs (1 MIC and 2 MIC) had an influence on the sensory characteristics of the finished products that may hinder their future applicability to improve the shelf-life of meat products. Therefore, further studies are required to clarify such an issue.
Open Access: Yes
DOI: 10.3303/CET23107043
Application of whey protein-based edible coatings containing lemon peel powder and extract to maintain the antioxidant properties of table grapes during ambient storage
Publication Name: Food and Humanity
Publication Date: 2025-12-01
Volume: 5
Issue: Unknown
Page Range: Unknown
Description:
Table grapes are among the most widely consumed fruits worldwide; however, their shelf life is limited by water loss, microbial spoilage, and degradation of antioxidants. This study examined the effects of whey protein-based edible coatings enriched with 1 % lemon peel powder (LP1), 2 % lemon peel powder (LP2), 1 % lemon peel extract (LE1), or 2 % lemon peel extract (LE2) on the quality of Red Globe table grapes during 14-day ambient storage. The highest weight loss (33.2 %) occurred in uncoated samples, while LP1 had the lowest (20.4 %), indicating improved moisture retention. The initial pH of uncoated table grapes (3.63) increased to 4.23 by the end of storage. All coatings slowed this increase, resulting in final pH values ranging from 4.03 to 4.10. Regarding antioxidant-related parameters, LP1 showed higher total polyphenol content (TPC), ascorbic acid content, antioxidant activity (DPPH assay), and total monomeric anthocyanin (TMA) by 43.7 %, 25.0 %, 24.1 %, and 10.1 %, respectively, compared to uncoated samples. Among extract-enriched coatings, only LE1 maintained significantly higher antioxidant activity (75.8 %), while TPC and ascorbic acid levels were comparable to those of the uncoated samples. TMA content in LE-treated table grapes (23.6–22.5 mg CGE/100 g) was lower than in uncoated samples (26.7 mg CGE/100 g). Multivariate analyses (PCA, HCA) revealed distinct clustering between coated and uncoated samples, with LP1 showing the most pronounced separation. These results indicate that LP1 treatment may help reduce weight loss and support antioxidant stability, offering a potentially sustainable postharvest strategy for table grapes.
Open Access: Yes
Assessing the texture profile and optimizing the temperature and soaking time for the rehydration of hot air-dried Auricularia auricula-judae mushrooms
Publication Name: Discover Food
Publication Date: 2025-12-01
Volume: 5
Issue: 1
Page Range: Unknown
Description:
Rehydrating dried jelly ear mushrooms allows them to take on the original shape, and texture, but no thorough study has been done to date to determine the ideal rehydration parameters. The study aimed to optimize the rehydration conditions of the hot-air-dried jelly ear mushroom, to achieve the most similar stock to the fresh mushroom. To achieve this, the mushrooms dried to a constant weight at 40 °C were soaked in water that had been heated to 20–100 °C for 10–70 min. The mushrooms were weighed and examined the texture profile to determine the rehydration %, hardness, gumminess, chewiness, springiness, and cohesiveness at each tested temperature and soaking time. The fresh mushroom used as a control had a moisture content of 95.39 m/m%, hardness of 847.40, springiness of 0.70, gumminess of 562.04, chewiness of 423.98 N/m2, and cohesiveness of 0.66 J/m3. These results were compared to the rehydrated mushroom samples texture profile test results, and it was found that the dried mushrooms recovered nearly the same texture as the fresh mushrooms with a 20-minute soak at 40 °C. As consumers prefer rehydrated products to be similar to fresh products in terms of texture and enjoyment value, it is crucial to determine the ideal rehydration parameters. However, each drying method and temperature has a different effect on the texture and water absorption capacity of the mushrooms, so the mentioned results are only achieved with the described parameters.
Open Access: Yes
