Klaudia Szalai

57189850594

Publications - 8

Quantitative comparison of some faecal bacterial communities in groups of Mangalica and commercial pigs

Károly Tempfli László Pongrácz Klaudia Szalai Sárvári Loretta Csilla Emil Balázs Herceg Ágnes Bali Papp

Publication Name: Bio Web of Conferences

Publication Date: 2024-08-23

Volume: 125

Issue: Unknown

Page Range: Unknown

Description:

Different housing technology, breed, age and nutrition can contribute to changes in the composition of microbial communities in pigs. Faecal samples from groups of Mangalica and commercial pigs were collected and analysed by qPCR in order to identify changes and differences regarding the quantity of total faecal bacteria, Prevotella genus, Lactobacillus spp., Bifidobacterium spp., Enterococcus spp. and the family Enterobacteriaceae. In both Mangalica and commercial pig samples, quantities of total faecal bacteria increased from weaner pigs to lactating sows. The relative quantity of total bacteria was larger (p<0.05) in Mangalica growers and lactating sows compared to commercial pigs. The ratio of Prevotella genus in total bacteria was higher (p<0.05) in Mangalica growers and lower in Mangalica lactating sows compared to respective commercial groups. The ratio of Lactobacillus spp. was largest (p<0.05) in samples of Mangalica boars, whereas ratios of Bifidobacterium spp. were greater (p<0.05) in Mangalica weaners, growers, and boars. Faecal samples of Mangalica growers contained a higher ratio of Enterobacteriaceae in total bacteria, whereas Enterococcus spp. was more prevalent in commercial weaner pigs and boars (p<0.05). Considerable changes in faecal bacteria communities were observed in association with different age and utilization.

Open Access: Yes

DOI: 10.1051/bioconf/202412503005

Effects of a Probiotic Supplement on the Quantity of Some Bacterial Communities in Fecal Samples of Lactating Sows

Tamás Tóth Viktor J. Vojnich Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Sárvári Loretta Csilla Botond Alpár

Publication Name: Chemical Engineering Transactions

Publication Date: 2024-01-01

Volume: 114

Issue: Unknown

Page Range: 949-954

Description:

Complex adaptation strategies concerning nutrition, housing technology, and veterinary treatment are required to maintain current production levels under increasingly stringent regulations on the preventive application of antibiotics. The reduced application of antibiotics is recommended for the sustainability of industrial pig production. Probiotic supplementation may contribute to improved sow and piglet health, mitigating the need for antibiotics. The effects of probiotic supplementation on sow performance and the quantity of fecal bacterial communities in lactating sows were investigated. Experimental sows received probiotic supplementation (n=10) and were compared to control sows (n=10). Fecal samples were collected from 20 sows in the second week of lactation. The quantitative measurement of total bacteria, Prevotella genus, Lactobacillus spp., and Bifidobacterium spp. was done by qPCR. Differences in backfat thickness (BFT), BFT loss, and feed intake of control and experimental sows were not significant (p>0.05). The amount of total bacteria, Prevotella, and Lactobacillus spp. was lower (p<0.05) in the fecal samples of experimental sows. The Prevotella percentage in total bacteria decreased, whereas Bifidobacterium spp. the ratio increased in experimental supplemented sows. Overall, probiotic supplementation resulted in notable alterations regarding some of the analyzed bacterial communities.

Open Access: Yes

DOI: 10.3303/CET24114159

Gene Expression of Peroxisome Proliferator-Activated Receptor γ in Mangalica and Large White Pigs

Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Sárvári Loretta Csilla Éva Rampasek

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 271-276

Description:

The expression of peroxisome proliferator-activated receptor γ gene (PPARγ) – a major regulator of adipogenesis and intramuscular fat deposition in pigs – was analysed in different tissues of Blonde Mangalica (M) and Hungarian Large White (LW) by means of quantitative reverse transcription PCR. In total, 20 experimental animals were raised and fed under identical conditions, and transported to the abattoir at similar body weight (124.7±10.3 kg; p>0.05). In both breeds highest PPARγ expression was observed in backfat (p<0.001), whereas lower levels were measured in muscle samples. The PPARγ mRNA levels in loin (m. longissimus dorsi) and semimembranosus muscle samples did not differ (p>0.05). Elevated PPARγ expression was observed in M backfat compared to LW (p<0.05). Loin and semimembranosus muscle samples of M showed higher PPARγ mRNA levels than LW (P<0.001), as well. Loin muscle marbling scores significantly correlated with β-actin normalized PPARγ expression (r=0.63; p<0.001) indicating the involvement of PPARγ in the regulation of intramuscular fat development. In conclusion, the differential expression of PPARγ in backfat and muscle samples can contribute to the remarkable differences in the constitution and intramuscular fat accumulation of fat-type M and lean-type commercial LW. Muscle PPARγ mRNA levels may be used as an indicator of marbling characteristics in pigs.

Open Access: Yes

DOI: 10.3303/CET23107046

Yield Performance, Laying Behaviour Traits and Egg Quality of a Crossbred Laying Hen in Alternative Housing Systems

Eszter Zsédely Károly Tempfli Klaudia Szalai Erika Lencsés-Varga G. Takács Sárvári Loretta Csilla

Publication Name: Chemical Engineering Transactions

Publication Date: 2023-01-01

Volume: 107

Issue: Unknown

Page Range: 121-126

Description:

One aim of this study was to investigate a crossbred laying hen line (400 hens) in two alternative housing systems in two replicates between 20-72 weeks of age. One rearing system was deep litter (D) with artificial light. Hens from Group R were housed in a poultry house with windows on deep litter and access to a runway without artificial light. Birds were fed ad libitum with a concentrate layer feed (18.0 % protein, 11.6 MJ). The egg production was influenced by the housing system. Lower average egg weight was observed in Group R. The feed consumption was also lower here, but the FCR was better compared to D. Floor egg did not cause any problem in the examined systems. The shell strength decreased with age in both systems. However, the rate was higher in Group D. The albumen height and HU value were higher in Group R at the end of the experiment (72nd week of age). Some differences were found in the fatty acid profile between the two housing systems. Another goal of the study was to observe the behaviour patterns of animals kept under different conditions. Behavioural traits were monitored for 100 hens in total on the two farms that applied different housing technologies (50-50 birds with or without outdoor access). Birds with outdoor access showed 0.23±0.81 aggression-related traits per hour, whereas chickens without outdoor access produced 0.15±0.76 of the same traits (p>0.05). Hens with outdoor access showed 2.30±4.65 activity-related traits, whereas, for hens without outdoor access, 0.72±1.83 observations were recorded per hour (p<0.05). Comfort behaviour was similar (p>0.05) in the two groups. In conclusion, the housing system has a complex effect on the assessed crossbred laying hen line.

Open Access: Yes

DOI: 10.3303/CET23107021

Dietary Inclusion of Defatted Silkworm (Bombyx mori L.) Pupa Meal for Broiler Chickens at Different Ages: Growth Performance, Carcass and Meat Quality Traits

Eszter Zsédely Marco Cullere Klaudia Szalai Yazavinder Singh Antonella Dalle Zotte G. Takács Zsolt Herman

Publication Name: Animals

Publication Date: 2023-01-01

Volume: 13

Issue: 1

Page Range: Unknown

Description:

The present study was conducted to assess the effects of a 4% defatted silkworm (SWM-DEF) meal dietary incorporation into chickens’ diet at different growth stages on growth performances, carcass, and meat quality traits. A total of 90 Ross 308 one-day-old male broiler chickens were randomly allocated into 3 dietary groups of 5 replicated pens/diet (6 chickens/pen). One group was fed a standard soybean-based diet (C); group SWM1 consumed a starter diet (1–10 days of age) including 4% SWM-DEF and then the C diet up to slaughter (11–42 days of age); group SWM2 was fed with the C diet in the starter phase and the 4% SWM-DEF diet up to slaughter. Individual live weight and pen feed intake were determined at the end of each feeding phase: starter, grower, and finisher. Weight gain and FCR were then calculated. At 42 days of age, chickens were slaughtered and carcass traits determined. Leg and breast physico-chemical meat quality was also evaluated. Results confirmed that SWM-DEF could be a possible alternative feed source for chickens since growth performance, carcass, and meat physical traits were overall comparable in the three treatment groups. The feeding stage seemed to play a relevant impact on the sole meat protein content (SWM1 < SMW2 and C; p < 0.001). Concluding, the results available to date indicate that 4% SWM-DEF can be provided to chickens in different growth phases, and the choice of the inclusion period is more an industrial cost-benefit evaluation. From the scientific point of view, however, an administration in the grower-finisher phase rather than in the starter one provided the best meat nutritional quality. Further investigations should focus on the impact on meat fatty acids profile and sensory traits, which are of utmost importance for consumers.

Open Access: Yes

DOI: 10.3390/ani13010119

Linseed oil supplementation affects fatty acid desaturase 2, peroxisome proliferator activated receptor gamma, and insulin-like growth factor 1 gene expression in turkeys (Meleagris gallopavo)

Eszter Zsédely E. Lakatos Károly Tempfli Klaudia Szalai András Gáspárdy Ágnes Bali Papp

Publication Name: Animal Bioscience

Publication Date: 2021-04-01

Volume: 34

Issue: 4

Page Range: 662-669

Description:

Objective: Effects of linseed oil (LO) supplementation on the fat content and fatty acid profile of breast meat, and the expression of three genes in the liver, breast muscle and fat tissues of commercial 154-day-old hybrid male turkeys were investigated. Methods: The animals in the control group were fed a commercially available feed and received no LO supplementation (n = 70), whereas animals in the LO group (n = 70) were fed the same basic diet supplemented with LO (day 15 to 21, 0.5%; day 22 to 112, 1%). The effect of dietary LO supplementation on fatty acid composition of breast muscle was examined by gas chromatography, and the expression of fatty acid desaturase 2 (FADS2), peroxisome proliferator activated receptor gamma (PPARγ), and insulin-like growth factor 1 (IGF1) genes was analysed by means of quantitative reverse transcription polymerase chain reaction. Results: The LO supplementation affected the fatty acid composition of breast muscle. Hepatic FADS2 levels were considerably lower (p<0.001), while adipose tissue expression was higher (p<0.05) in the control compared to the LO group. The PPARγ expression was lower (p<0.05), whereas IGF1 was higher (p<0.05) in the fat of control animals. There were no significant (p>0.05) differences in FADS2, PPARγ, and IGF1 gene expressions of breast muscle; however, omega-6/omega-3 ratio of breast muscle substantially decreased (p<0.001) in the LO group compared to control. Conclusion: Fatty acid composition of breast meat was positively influenced by LO supplementation without deterioration of fattening parameters. Remarkably, increased FADS2 expression in the liver of LO supplemented animals was associated with a significantly decreased omega-6/omega-3 ratio, providing a potentially healthier meat product for human consumption. Increased PPARγ expression in fat tissue of the LO group was not associated with fat content of muscle, whereas a decreased IGF1 expression in fat tissue was associated with a trend of decreasing fat content in muscle of the experimental LO group.

Open Access: Yes

DOI: 10.5713/ajas.20.0030

Genotyping of four loci in Hungarian yellow and broiler chickens

Károly Tempfli Klaudia Szalai Erika Lencsés-Varga Ágnes Bali Papp

Publication Name: Acta Veterinaria Hungarica

Publication Date: 2019-01-01

Volume: 67

Issue: 1

Page Range: 1-10

Description:

Traditional selection has led to remarkable differences in allele frequencies among various chicken breeds. Indigenous and broiler-type chicken populations were genotyped for polymorphisms in thyroid hormone responsive Spot14α, prolactin (PRL), IGF-binding protein 2 (IGFBP2), and somatostatin (SST) genes in order to determine potential utilisation type-associated allele frequencies. Significant (P < 0.05) differences were detected between Hungarian Yellow and broiler populations for Spot14α, PRL, and IGFBP2 allele frequencies, whereas the same SST allele (A) was fixed in both groups. In this study, the most significant associations (P < 0.05) were found between the IGFBP2 genotypes and the measured traits (body weight, carcass weight, breast muscle weight with or without skin, breast muscle weight as a percentage of carcass weight) in the broiler population. The results can be applied for the evaluation of polymorphism effects in the analysed populations; however, contradictory allele effects in different breeds and hybrids indicate the need for cautious marker utilisation in selection programmes.

Open Access: Yes

DOI: 10.1556/004.2019.001

Differential expression of six genes in fat-type Hungarian Mangalica and other pigs

Zoltán Simon Károly Tempfli László Pongrácz Barbara Kiss Klaudia Szalai Ágnes Bali Papp

Publication Name: Archives Animal Breeding

Publication Date: 2016-06-14

Volume: 59

Issue: 2

Page Range: 259-265

Description:

In order to identify potential variances in gene expression of phenotypically different pig breeds, six fat-metabolism-related genes were analyzed in backfat and muscle tissues of fat-type Mangalica (MAN), Mangalica x Duroc (MD), and lean-type Hungarian Large White (HLW) and Pietrain x Duroc (PD) pigs by means of quantitative reverse transcription PCR (qRT-PCR). Higher (P < 0.05) adipocyte fatty-acid-binding protein (A-FABP) expression was observed in backfat and muscle tissues of purebred and crossbred MAN than in those of HLW and PD. In all breeds and crosses, adiponectin (ADIPOQ) was predominantly expressed in backfat at a similar level (P > 0.05), whereas muscle ADIPOQ expression was highest (P < 0.05) in MAN and MD. Levels of fatty acid synthase (FASN) mRNA were greatest in MAN, moderate in MD, and lowest in HLW and PD backfat and muscle. The fat mass and obesity-associated gene (FTO) was more abundant in MAN and MD backfat, whereas muscle expressions did not differ (P > 0.05) between breeds. Regarding leptin (LEP) expression, MAN produced the greatest levels in backfat, while HLW produced the lowest. In muscle, highest LEP was detected in MAN and MD. Between groups, perilipin 2 (PLIN2) was expressed similarly in backfat; however, PLIN2 was more abundant in muscle of MAN and MD than in that of HLW and PD. Differences in gene expression can contribute to the development of the characteristic fatty phenotype in MAN pigs. The identification of differentially expressed genes facilitates targeted sequencing and genotyping efforts for further studies.

Open Access: Yes

DOI: 10.5194/aab-59-259-2016