Dóra Pinczés

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Publications - 7

Viral coat proteins decrease the gene silencing activity of cognate and heterologous viral suppressors

László Palkovics Katalin Nemes Réka Sáray Dóra Pinczés Katalin Salánki

Publication Name: Scientific Reports

Publication Date: 2024-12-01

Volume: 14

Issue: 1

Page Range: Unknown

Description:

Plant viruses have evolved different viral suppressors of RNA silencing (VSRs) to counteract RNA silencing which is a small RNA-mediated sequence-specific RNA degradation mechanism. Previous studies have already shown that the coat protein (CP) of cucumber mosaic virus (CMV) reduced RNA silencing suppression (RSS) activity of the VSR of CMV, the 2b protein. To demonstrate the universality of this CP-VSR interference, our study included three different viruses: CMV and peanut stunt virus (PSV) from the Bromoviridae, and plum pox virus (PPV) from the Potyviridae family. The RSS activity of the three VSRs (CMV 2b, PSV 2b, and PPV HC-Pro) was compared using Agrobacterium-mediated transient expression in Nicotiana benthamiana and the effect of CMV CP, PSV CP and PPV CP was validated on the RSS activity of their cognate and heterologous VSRs as well. Furthermore, the VSRs were also evaluated in PTGS suppressor-deficient CMV mutant (CMV NVE/10–12/AAA) virus-infected plants. The joint presence of CPs and VSRs resulted in decreased RSS activity in each combination, regardless of the origin of the two proteins, suggesting a universal role of the viral CPs in fine tuning of RSS. Interestingly the PSV CP elicited the strongest negative effect on the RSS activity of all three VSRs.

Open Access: Yes

DOI: 10.1038/s41598-024-81998-4

Biology, phylogenetic and evolutionary relations of Tradescantia mild mosaic virus isolates from Hungary

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Journal of Plant Pathology

Publication Date: 2024-08-01

Volume: 106

Issue: 3

Page Range: 1369-1374

Description:

In Western Transdanubia in 2018 and in Central-Hungary in 2022 spiderwort plants showed flower breaking symptoms and mild mosaic on the foliage, which indicated a potential virus infection. One gram of symptomatic leaf sample was collected at both locations. Potyvirus-specific ELISA tests demonstrated potyvirus infection. To identify the virus species, potyvirus-specific RT-PCR was carried out on the samples. In both samples specific PCR products were detected and cloned into pGEM®-T Easy vector. The nucleotide sequences of the inserts were determined by Sanger sequencing. BLASTn searches on the complete coat protein region of both isolates demonstrated more than 99.87% identity with Tradescantia mild mosaic virus (TraMMV; accession number OL584375). Koch postulates were fulfilled by sap inoculating seed grown spiderwort plants. Phylogenetic analyses of the TraMMV coat protein sequences revealed two distinct evolutionary lineages: a tropical subgroup with at least 97.84% identity within the group and temperate subgroup with at least 98.97% identity within the group. One major difference between the subgroups was in the triplet responsible for vector transmission. The isolates belonging to the tropical subgroup had DAG triplets, while the temperate subgroup had NAG triplets. The difference in the triplets could be caused by natural diversification, directional selection or disruptive selection. License: CC BY-NC-ND

Open Access: Yes

DOI: 10.1007/s42161-024-01661-9

First Report of Saffron Latent Virus in Crocus sativus from Hungary

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Plant Disease

Publication Date: 2024-02-01

Volume: 108

Issue: 2

Page Range: 540

Description:

No description provided

Open Access: Yes

DOI: 10.1094/PDIS-09-23-1765-PDN

Genetic variability of grapevine Pinot gris virus (GPGV) in an organically cultivated vineyard in Hungary

Tamás DEÁK Erzsébet SZATHMÁRY László Palkovics Réka Sáray Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Phytopathologia Mediterranea

Publication Date: 2024-01-01

Volume: 63

Issue: 2

Page Range: 179-190

Description:

Grapevine Pinot gris virus (GPGV) is a recently identified trichovirus infecting grapevines. Despite wide distribution, there is limited available information on epidemiology, transmission, and associated symptoms of grapevine leaf mottling and deformation. Occurrence and genetic diversity of GPGV variants were surveyed in an organically cultivated Hungarian vineyard that was planted between 1996 and 2014. Sequence analysis demonstrated the widespread presence and high variability of GPGV, and according to phylogenetic analyses, the Hungarian virus isolates were classified into three groups. Most of the identified variants clustered with the representative asymptomatic isolates, but all isolates from one grapevine cultivar grouped with representative isolates of clade B. Furthermore, one isolate clustered with representative isolates of clade C, and the identified clade C variant had previously undescribed polymorphisms.

Open Access: Yes

DOI: 10.36253/phyto-14492

First Report of Meadow Saffron Breaking Virus on Wild Colchicum autumnale from a Strictly Protected Natura2000 Site at a Hungarian National Park

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Plant Disease

Publication Date: 2023-06-01

Volume: 107

Issue: 6

Page Range: 1955

Description:

No description provided

Open Access: Yes

DOI: 10.1094/PDIS-08-22-1787-PDN

Thalia dealbata, a new host of sugarcane mosaic virus

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Journal of Plant Pathology

Publication Date: 2023-05-01

Volume: 105

Issue: 2

Page Range: 587-588

Description:

No description provided

Open Access: Yes

DOI: 10.1007/s42161-022-01295-9

Peanut stunt virus movement protein is the limiting factor in Capsicum annuum infection

László Palkovics Attila Fábián Dóra Pinczés Katalin Salánki

Publication Name: Virus Research

Publication Date: 2022-10-02

Volume: 319

Issue: Unknown

Page Range: Unknown

Description:

Cucumber mosaic virus (CMV) is one of the most devastating plant viruses, with more than 1,200 species of host plants. The host range and economic importance of peanut stunt virus (PSV) are mostly limited to legumes, despite the similar taxonomy and genome structure with CMV. Since no data are available on the background of the limited host range of PSV, RNA 3 recombinant and reassortant viruses were generated (C12P3, P12C3, C12CP3, C12PC3, C12PΔC3) to study their infection phenotype on a common host (Nicotiana benthamiana) and on a selective host (Capsicum annuum cv. Brody). The PSV movement protein (MP) was not able to function with the coat protein (CP) of CMV unless the C-terminal 42 amino acids were deleted from the PSV MP. As a result of the inoculation experiments, MP was considered the protein influencing symptom phenotypes on N. benthamiana and responsible for the host range difference on the pepper. Since plasmodesmata (PD) localization of viral MPs is essential for cell-to-cell movement, subcellular localization of GFP-tagged MPs (CMV-MP-eGFP, PSV-MP-eGFP) was observed. In the case of CMV-MP-eGFP, clear colocalization with PD was detected in both hosts, but PSV-MP-eGFP was not tightly connected to the PD in N. benthamiana and barely localized to the PD in C. annuum epidermal cells. Measuring Pearson correlation coefficients (PCCs) also supported the visual observation.

Open Access: Yes

DOI: 10.1016/j.virusres.2022.198879