Asztéria Almási

6602311582

Publications - 6

Biology, phylogenetic and evolutionary relations of Tradescantia mild mosaic virus isolates from Hungary

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Journal of Plant Pathology

Publication Date: 2024-08-01

Volume: 106

Issue: 3

Page Range: 1369-1374

Description:

In Western Transdanubia in 2018 and in Central-Hungary in 2022 spiderwort plants showed flower breaking symptoms and mild mosaic on the foliage, which indicated a potential virus infection. One gram of symptomatic leaf sample was collected at both locations. Potyvirus-specific ELISA tests demonstrated potyvirus infection. To identify the virus species, potyvirus-specific RT-PCR was carried out on the samples. In both samples specific PCR products were detected and cloned into pGEM®-T Easy vector. The nucleotide sequences of the inserts were determined by Sanger sequencing. BLASTn searches on the complete coat protein region of both isolates demonstrated more than 99.87% identity with Tradescantia mild mosaic virus (TraMMV; accession number OL584375). Koch postulates were fulfilled by sap inoculating seed grown spiderwort plants. Phylogenetic analyses of the TraMMV coat protein sequences revealed two distinct evolutionary lineages: a tropical subgroup with at least 97.84% identity within the group and temperate subgroup with at least 98.97% identity within the group. One major difference between the subgroups was in the triplet responsible for vector transmission. The isolates belonging to the tropical subgroup had DAG triplets, while the temperate subgroup had NAG triplets. The difference in the triplets could be caused by natural diversification, directional selection or disruptive selection. License: CC BY-NC-ND

Open Access: Yes

DOI: 10.1007/s42161-024-01661-9

First Report of Saffron Latent Virus in Crocus sativus from Hungary

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Plant Disease

Publication Date: 2024-02-01

Volume: 108

Issue: 2

Page Range: 540

Description:

No description provided

Open Access: Yes

DOI: 10.1094/PDIS-09-23-1765-PDN

Genetic variability of grapevine Pinot gris virus (GPGV) in an organically cultivated vineyard in Hungary

Tamás DEÁK Erzsébet SZATHMÁRY László Palkovics Réka Sáray Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Phytopathologia Mediterranea

Publication Date: 2024-01-01

Volume: 63

Issue: 2

Page Range: 179-190

Description:

Grapevine Pinot gris virus (GPGV) is a recently identified trichovirus infecting grapevines. Despite wide distribution, there is limited available information on epidemiology, transmission, and associated symptoms of grapevine leaf mottling and deformation. Occurrence and genetic diversity of GPGV variants were surveyed in an organically cultivated Hungarian vineyard that was planted between 1996 and 2014. Sequence analysis demonstrated the widespread presence and high variability of GPGV, and according to phylogenetic analyses, the Hungarian virus isolates were classified into three groups. Most of the identified variants clustered with the representative asymptomatic isolates, but all isolates from one grapevine cultivar grouped with representative isolates of clade B. Furthermore, one isolate clustered with representative isolates of clade C, and the identified clade C variant had previously undescribed polymorphisms.

Open Access: Yes

DOI: 10.36253/phyto-14492

First Report of Meadow Saffron Breaking Virus on Wild Colchicum autumnale from a Strictly Protected Natura2000 Site at a Hungarian National Park

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Plant Disease

Publication Date: 2023-06-01

Volume: 107

Issue: 6

Page Range: 1955

Description:

No description provided

Open Access: Yes

DOI: 10.1094/PDIS-08-22-1787-PDN

Thalia dealbata, a new host of sugarcane mosaic virus

László Palkovics Réka Sáray János Ágoston Dóra Pinczés Asztéria Almási Katalin Salánki

Publication Name: Journal of Plant Pathology

Publication Date: 2023-05-01

Volume: 105

Issue: 2

Page Range: 587-588

Description:

No description provided

Open Access: Yes

DOI: 10.1007/s42161-022-01295-9

Sternbergia lutea, a new host of Narcissus late season yellows virus

László Palkovics János Ágoston Asztéria Almási Katalin Salánki

Publication Name: Phytopathologia Mediterranea

Publication Date: 2021-01-01

Volume: 60

Issue: 3

Page Range: 403-407

Description:

In autumn 2017, autumn daffodil plants with yellow-green stripes on the leaves were observed at a botanical garden in Budapest, Hungary. Indicator plants wereinoculated, but symptoms did not develop. RT-PCR tests of the indicator plants werealso negative for the viruses. Potyvirus specific ACP-ELISA and RT-PCR were carriedout on the symptomatic S. lutea leaf samples. RT-PCR with universal potyvirusprimers resulted in one, approx. 1700 base pair PCR product. Phylogenetic analysis ofthe nucleotide sequence of the coat protein demonstrated 98.78-99.51% identity withthree Japanese isolates of Narcissus late season yellows virus. While unidentified potyvirusinfection of autumn daffodil has been previously reported, sequence data havenot been published. Therefore, this is the first report of Sternbergia lutea as a host ofNarcissus late season yellows virus

Open Access: Yes

DOI: 10.36253/phyto-12709