In autumn 2017, autumn daffodil plants with yellow-green stripes on the leaves were observed at a botanical garden in Budapest, Hungary. Indicator plants wereinoculated, but symptoms did not develop. RT-PCR tests of the indicator plants werealso negative for the viruses. Potyvirus specific ACP-ELISA and RT-PCR were carriedout on the symptomatic S. lutea leaf samples. RT-PCR with universal potyvirusprimers resulted in one, approx. 1700 base pair PCR product. Phylogenetic analysis ofthe nucleotide sequence of the coat protein demonstrated 98.78-99.51% identity withthree Japanese isolates of Narcissus late season yellows virus. While unidentified potyvirusinfection of autumn daffodil has been previously reported, sequence data havenot been published. Therefore, this is the first report of Sternbergia lutea as a host ofNarcissus late season yellows virus