Judit Hohmann

7004457196

Publications - 4

Jacaranone Derivatives with Antiproliferative Activity from Crepis pulchra and Relevance of This Group of Plant Metabolites

Gy Pinke Norbert Kúsz Ágnes Kulmány Csilla Zsuzsanna Dávid István Zupkó Andrea Vasas Judit Hohmann

Publication Name: Plants

Publication Date: 2022-03-01

Volume: 11

Issue: 6

Page Range: Unknown

Description:

Jacaranones are a small group of specific plant metabolites with promising biological activi-ties. The occurrence of jacaranones is limited to only a few plant families, with Asteraceae being the most abundant source of these compounds. Therefore, jacaranones can also serve as chemotaxonomic markers. Our phytochemical investigation of Crepis pulchra L. (Asteraceae) resulted in three jacara-none derivatives (jacaranone, 2,3-dihydro-2-hydroxyjacaranone, 2,3-dihydro-2-methoxyjacaranone), and (6R,9S)-3-oxo-α-ionol-β-D-glucopyranoside, fulgidic acid, 12,15-octadecadienoic acid methyl es-ter, scopoletin and apigenin-7-O-β-D-glucoside. This is the first report on the isolation of jacaranones from a species belonging to the Cichorioideae subfamily of Asteraceae. Jacaranone derivatives were subjected to an in vitro antiproliferative assay against a panel of human cancer cell lines (MCF-7, MDA-MB-231, HeLa, and C33A), revealing high or moderate activities, with IC50 values ranging from 6.3 to 26.5 µM.

Open Access: Yes

DOI: 10.3390/plants11060782

Isolation of chemical constituents from Filago vulgaris and antiproliferative activity of the plant extract and its flavonoid against human tumor cell lines

G. Király Gy Pinke István Zupkó Zsuzsanna Hajdú Peter Forgo Judit Hohmann

Publication Name: Pakistan Journal of Pharmaceutical Sciences

Publication Date: 2020-07-01

Volume: 33

Issue: 4

Page Range: 1593-1597

Description:

Phytochemical investigation of the whole plant of Filago vulgaris Lam. (Asteraceae) resulted in the isolation and characterization of seven compounds, including a rare methoxylated flavonol (araneol), tetrahydrofurofuranolignans (pinoresinol and syringaresinol), p-hydroxybenzaldehyde, vanillin, vanillic acid and scopoletin. The structures of the compounds were determined by NMR and mass spectroscopy. All compounds were first obtained from this species and reported for the genus Filago. Our results demonstrate that highly methoxylated flavonols lacking substituents on ring B and lignans can be regarded as taxonomic markers for the tribe Inuleae. The lipophilic extract of F. vulgaris was found to have antiproliferative activity against HeLa cells (62.1±0.9% inhibition at 30 µg/ml), and araneol was highly effective against this tumour cell line (IC50 8.36 μM).

Open Access: Yes

DOI: 10.36721/PJPS.2020.33.4.REG.1593-1597.1

Anti-inflammatory activity of melampyrum barbatum and isolation of iridoid and flavonoid compounds

Anita Sztojkov-Ivanov G. Király Edit Wéber Gy Pinke Laura Fási Judit Hohmann Róbert Gáspár Erzsébet Háznagy-Radnai

Publication Name: Natural Product Communications

Publication Date: 2018-03-01

Volume: 13

Issue: 3

Page Range: 235-236

Description:

Melampyrum barbatum Waldst. & Kit. Ex Willd. (Scrophulariaceae) has been used in traditional medicine for the treatment of rheumatic complaints and different skin diseases. In the course of our study the anti-inflammatory activity of the aerial parts of M. Barbatum was evaluated. A MeOH extract was prepared and consecutively partitioned with CHCl3, EtOAc and n-BuOH. The fractions were assayed in in vivo carrageenan-induced rat paw oedema model. The intraperitoneally administered n-BuOH phase exerted marked inhibitory effect (33.6 %, p < 0.01). Multistep chromatographic separation afforded mussaenoside and aucubine from n-BuOH fraction. Moreover, 8-epiloganin, loganic acid and mussaenoside were obtained from EtOAc fraction and apigenin, luteolin, benzoic acid and galactitol from CHCl3 fraction. These data validate the ethnomedicinal use of M. Barbatum for the treatment of inflammatory diseases and reveal that iridoids and flavonoids could be responsible for the anti-inflammatory effect of this species.

Open Access: Yes

DOI: 10.1177/1934578x1801300301

Myrsinane, Premyrsinane, and Cyclomyrsinane Diterpenes from Euphorbia falcata as Potassium Ion Channel Inhibitors with Selective G Protein-Activated Inwardly Rectifying Ion Channel (GIRK) Blocking Effects

Attila Csorba Péter Orvos Gy Pinke László Tálosi Andrea Vasas Peter Forgo Judit Hohmann

Publication Name: Journal of Natural Products

Publication Date: 2016-08-26

Volume: 79

Issue: 8

Page Range: 1990-2004

Description:

GIRK channels are activated by a large number of G protein-coupled receptors and regulate the electrical activity of neurons, cardiac atrial myocytes, and β-pancreatic cells. Abnormalities in GIRK channel function have been implicated in the pathophysiology of neuropathic pain, drug addiction, and cardiac arrhythmias. In the heart, GIRK channels are selectively expressed in the atrium, and their activation inhibits pacemaker activity, thereby slowing the heart rate. In the present study, 19 new diterpenes, falcatins A-S (1-19), and the known euphorprolitherin D (20) were isolated from Euphorbia falcata. The compounds were assayed on stable transfected HEK-hERG (Kv11.1) and HEK-GIRK1/4 (Kir3.1 and Kir3.4) cells. Blocking activity on GIRK channels was exerted by 13 compounds (61-83% at 10 μM), and, among them, five possessed low potency on the hERG channel (4-20% at 10 μM). These selective activities suggest that myrsinane-related diterpenes are potential lead compounds for the treatment of atrial fibrillation.

Open Access: Yes

DOI: 10.1021/acs.jnatprod.6b00260