Introduction: Professionals working in the social sector typically do significant overwork in rather unfavourable working conditions. Although the purpose in life is proved to determine the areas of career, health awareness, and the quality of life, the latter has not yet been confirmed among social workers. Objective: The purpose of this research is to demonstrate body composition and fitness status as well as the quality of life of female employees (n = 127) at a social institution, furthermore the decisive role purpose of life plays in these indicators. Method: Data were collected through questionnaires, tests, body composition analyses, and fitness status tests, and were assessed by t-test, analysis of variance and regression analysis. Results: Body composition of the participants indicates a generally overweight status, while fitness status, quality of life, and purpose of life are within the acceptable range, however, all with notable range values. Those with a higher-level purpose of life demonstrate better fitness indicators and higher quality of life. In terms of body composition, fitness, and quality of life, only a minor difference was found between those who do intellectual and physical type of work. Those who took sick leave demonstrated a lower level of fitness and quality of life compared to those who did not take any sick leave. Body composition and fitness status have proven to deteriorate with age. Conclusion: Previous research findings can be confirmed by stating that neither the body composition and fitness indicators, nor the quality-of-life level of social workers are optimal. The determining role of purpose in life has been proven in substantial aspects in this study. One of the focus areas of enhancing purpose in life is the workplace, where major positive impacts can be achieved in terms of the physical and mental health, well-being, and work performance.