The aim of the research is to investigate the relationship between motivation and organisational culture from the viewpont of employees working at SMEs in Slovakia. The study examines all of this during the COVID-19 period, which took a heavy toll on individuals’ social and organisational relationships as well. To investigate this, first the overlaps between the two areas were indentified. When exploring the theoretical part, the authors faced with the problem that the influence of motivation and organisational culture was usually examined together, and not on each other. A questionnaire survey was conducted, to which 581 valuable responses were received. Based on the results, it was proven that the wider the range of motivation tools used, the more positive the employees evaluate the organisational culture. All of this is worth considering for companies that would like to build a suitable organisational culture in their company.