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Publications - 6383

FIRST APPLICATION OF AN IMPROVED-RESOLUTION DARK-FIELD MICROSCOPY SETUP, COMBINED WITH A NOVEL DIRECT-STAINING LIVE IMMUNOFLUORESCENCE INVESTIGATION METHOD, VISUALISING THE MOTION OF BORRELIA BURGDORFERI SPIROCHETES

András P. Bózsik Béla P. Bózsik Béla P. Bózsik Béla P. Bózsik

Publication Name: Acta Microscopica

Publication Date: 2024-01-01

Volume: 33

Issue: 1

Page Range: 54-62

Description:

Dark-field microscopy has been increasingly applied in diagnostics in recent years, for example in Lyme borreliosis, due to the growing concerns about the diagnostic utility of standard serological tests. Although the diagnostic value of microscopic methods is still debated due to the lack of proper clinical testing and the definition of the diagnostic cutoff levels, it is important that the spirochetes causing the disease can be visualized from body fluids via dark-field investigation. Additionally, a specific identification of Borrelia is still not routinely possible, visual observation is usually fortified with additional PCR investigations. We are publishing the pictures from the first-time application of an enhanced dark-field illumination technique that improves the resolution and picture quality of digital recordings. The same illumination setup, combined with a novel live staining of Borrelia burgdorferi with cleaned, FITC-labelled anti-Borrelia antibodies, has produced remarkable recordings of the same spirochetes, in natural motion. All experiments were performed on remnant samples of DualDur kit laboratory investigations.

Open Access: Yes

DOI: DOI not available

Recombinant Potyvirus lilimaculae in asymptomatic Galanthus nivalis: Ecological and evolutionary implications

László Palkovics János Ágoston Asztéria Almási Katalin Salánki

Publication Name: Plos One

Publication Date: 2026-03-01

Volume: 21

Issue: 3 March

Page Range: Unknown

Description:

Snowdrops are economically important early-spring flowering geophytes. They are protected both in Hungary and across the European Union, and their international trade is regulated under the Washington Convention. Despite their importance, viro-logical research on Galanthus species and cultivars remains limited. To date, only a single virus, Snowdrop virus Y, a tentative member of the genus Potyvirus within the family Potyviridae, has been identified in Galanthus. In this study, ten wild leaf samples and one cultivated, asymptomatic leaf samples were collected in accordance with the prescribed permit conditions. Both ELISA and RT-PCR tests were conducted, indicating infection by a Potyvirus. Direct sequencing of the PCR products revealed nucleotide heterogeneity in one sample, suggesting infection with multiple isolates of the same virus. In this case, the purified PCR product was ligated into pGEM®-T Easy vector. Individual clones were then sequenced to identify the distinct viral isolates present. A BLAST analysis of the sequences revealed that all three snowdrop isolates shared at least 96% identity in the complete coat protein region with the Potyvirus lilimaculae (lilí mottle virus, LMoV) isolate Handan (JF714974), previously identified in Narcissus tazetta var. chinensis from Hubei Province, China. To confirm pathogenicity, sap inoculation was performed on ELISA- and RT-PCR-negative snowdrops. One year later, these plants were re-tested via the same methods, which confirmed successful infection. Phylogenetic analysis revealed that all snowdrop isolates clustered within subgroup II and presented strong recombination signals. The detection of LMoV in asymptomatic wild plants has major implications for nature conservation, horticulture, virus reservoir identification, phytosanitary regulation, and our understanding of Potyviridae evolution.

Open Access: Yes

DOI: 10.1371/journal.pone.0345337

Surface modification and wear properties of direct metal laser sintered hybrid tools used in moulds

Imre Fekete József Gábor Kovács Tamás Ibriksz Mária B. Maros István Hatos Hajnalka Hargitai

Publication Name: Journal of Mechanical Engineering

Publication Date: 2018-01-01

Volume: 64

Issue: 2

Page Range: 121-129

Description:

Injection moulding is one of the most productive plastic forming processes. Product development and the reduction of production time require new solutions in tooling design and manufacturing. Direct metal laser sintering (DMLS) allows moulds to be built with special cooling systems, which offer curved cooling lines that can follow the geometry of the part (conformal cooling). One disadvantage of DMLS, its high cost, can be dramatically reduced with the building of hybrid structures. With conventional tool steels as the base plate and only the special geometry of the part sintered on the top, the final geometry can be manufactured after sintering by conventional process technologies. We produced hybrid structures by direct metal laser sintering maraging steel (MS1) powder onto the surface of commercial mould steels and studied the effect of different heat treatments on porosity, tribological behaviour and the microstructure. The transition zone was also characterized.

Open Access: Yes

DOI: 10.5545/sv-jme.2017.4942

Pushover Analysis in Seismic Engineering: A Detailed Chronology and Review of Techniques for Structural Assessment

Orsolya Kegyes-Brassai Kevin Karanja Kuria

Publication Name: Applied Sciences Switzerland

Publication Date: 2024-01-01

Volume: 14

Issue: 1

Page Range: Unknown

Description:

This study analyzes the progression, utilization, and inherent challenges of traditional non-linear static procedures (NSPs) such as the capacity spectrum method, the displacement coefficient method, and the N2 method for evaluating seismic performance in structures. These methods, along with advanced versions such as multi-mode, modal, adaptive, and energy-based pushover analysis, help determine seismic demands, enriching our grasp on structural behaviors and guiding design choices. While these methods have improved accuracy by considering major vibration modes, they often fall short in addressing intricate aspects such as bidirectional responses, torsional effects, soil-structure interplay, and variations in displacement coefficients. Nevertheless, NSPs offer a more comprehensive and detailed analysis compared to rapid visual screening methods, providing a deeper understanding of potential vulnerabilities and more accurate predictions of structural performance. Their efficiency and reduced computational demands, compared to the comprehensive nonlinear response history analysis (NLRHA), make NSPs a favored tool for engineers aiming for swift seismic performance checks. Their accuracy and application become crucial when gauging seismic risks and potential damage across multiple structures. This paper underscores the ongoing refinements to these methods, reflecting the sustained attention they receive from both industry professionals and researchers.

Open Access: Yes

DOI: 10.3390/app14010151

Effect of GNSS Spoofing on GNSS-IMU Data Fusion-based Vehicle Pose Estimation

Péter Bauer

Publication Name: IFAC Papersonline

Publication Date: 2025-10-01

Volume: 59

Issue: 30

Page Range: 150-155

Description:

This paper examines the effect of GNSS spoofing on GNSS-IMU fusion-based position, velocity and attitude estimates. First, it proposes and tunes an estimator considering also IMU sensor biases and real flight data of a multicopter. Then it feeds the estimator with IMU data from a fixed flight trajectory and GNSS data from different trajectories with increasing divergence from the fixed one simulating a perfect spoofing scenario. Detailed examination of the estimates shows that spoofing has non negligible effect on velocity and especially attitude estimates. Thus any spoofing detection algorithm can not be based on attitude estimates which utilize GNSS data.

Open Access: Yes

DOI: 10.1016/j.ifacol.2025.12.228

Relationship between knowledge sharing willingness and life goals of generation Z

Renata Machova Andrea Bencsik Tímea Juhász Peter Molnar

Publication Name: Proceedings of the European Conference on Knowledge Management Eckm

Publication Date: 2018-01-01

Volume: 1

Issue: Unknown

Page Range: 84-94

Description:

The most critical area of knowledge management systems is the knowledge transfer and knowledge sharing. Several research results have emerged applying different approaches with a focus why employees and leaders of companies do not share their knowledge. Differences in social, cultural and individual values are in the background of willingness to share knowledge. Each of these attributes raise further questions. The main objective of this research is to examine the knowledge sharing willingness of Generation Z and consider the cultural determinants having impact on them. The research objective was to compare the results of Hungary and Slovakia. A questionnaire survey was conducted among the students of elementary and high schools. The electronic questionnaire included questions on a 5-point Likert scale, which were evaluated with a help of simple and complex statistical methods using the SPSS program. The study of life goals was based on the study of Aspiration Index in earlier research. The results show that cultural characteristics have significant influence on the characteristic features of behaviour related to trust, learning and knowledge. The national culture has impact on knowledge-friendly attitude of the school community. According to Aspiration Index Analysis, intrinsic goals gain more importance than extrincic goals. While getting older, the importance on intrinsic goals is increasing. The highly materialistic approach (preference of extrinsic goals) reduces the ability to coopearate. It increases competition and the self-interest, which are obstacles to knowledge-sharing. The ratio of goals (intrinsic/extrinsic) determines the decisions of young people (career choice), as well as those important skills that are relevant in the society and influence career choice e.g. cooperation, responsibility.

Open Access: Yes

DOI: DOI not available

First Successful Treatment Reported with Pembrolizumab in a Patient Diagnosed with Choriocarcinoma in Hungary

Vilmos Fülöp Kornél Lakatos László Kalmár Erzsébet Gyöngyvirág Bíró Erika Lahm

Publication Name: Life

Publication Date: 2026-03-01

Volume: 16

Issue: 3

Page Range: Unknown

Description:

Pembrolizumab is a programmed cell death protein (PD-1) inhibitor, humanized antibody widely used in cancer immunotherapy. Choriocarcinoma is an aggressive type of gestational trophoblastic neoplasia. Its treatment is based on surgical removal of the tumorous tissue and systemic chemotherapy; however, in some chemoresistant cases, immunotherapy can also be a valid option. Here, we report the first successful programmed death inhibitor-based treatment of a patient diagnosed with stage IV, ultra-high-risk choriocarcinoma in Hungary.

Open Access: Yes

DOI: 10.3390/life16030481

Innovative Cone Clustering and Path Planning for Autonomous Formula Student Race Cars Using Cameras †

Gergo Igneczi Balázs Szőnyi

Publication Name: Engineering Proceedings

Publication Date: 2024-01-01

Volume: 79

Issue: 1

Page Range: Unknown

Description:

In this research, we present a novel approach for cone clustering, path planning, and path visualization in autonomous Formula Student race cars, utilizing the YOLOv8 model and a ZED 2 camera, executed on a Jetson Orin computer. Our system first identifies and then deprojects the positions of cones in space, employing an advanced clustering mechanism to generate midpoints and draw connecting lines. In previous clustering algorithms, cones were stored separately by color and connected based on relevance to create the lane edges. However, our proposed solution adopts a fundamentally different approach. Cones on the left and right sides within a dynamically changing maximum and minimum distance are connected by a central line, and the midpoint of this line is marked distinctly. Cones connected in this manner are then linked by their positions to form the edges of the track. The midpoints on these central lines are displayed as markers, facilitating the visualization of the optimal path. In our research, we also cover the analysis of the clustering algorithm on global maps. The implementation utilizes the ROS 2 framework for real-time data handling and visualization. Our results demonstrate the system’s efficiency in dynamic environments, highlighting potential advancements in the field of autonomous racing. The limitation of our approach is the dependency on precise cone detection and classification, which may be affected by environmental factors such as lighting and cone positioning.

Open Access: Yes

DOI: 10.3390/engproc2024079096

Enabling industry symbiosis between energy-intensive industries via optimal integration of thermal energy storage

Ákos Orosz Viknesh Andiappan Bing Shen How Sin Yong Teng Tiffany Ang Ferenc Friedler

Publication Name: Thermal Science and Engineering Progress

Publication Date: 2026-06-01

Volume: 74

Issue: Unknown

Page Range: Unknown

Description:

Energy-based industrial symbiosis is a potential decarbonisation strategy for energy-intensive industries, which contribute significantly to carbon emissions. Thermal energy storage (TES) can be integrated to enhance energy efficiency and operational flexibility, while addressing issues related to supply–demand fluctuations. Nonetheless, the economic feasibility of TES-supported interplant heat recovery depends on the costs and properties of the storage media incorporated. Therefore, this work presents a systematic framework for optimising TES selection across a spectrum of storage options for interplant indirect heat integration. The objective is to minimise the total annualised cost (TAC), comprising energy and storage capital costs. The optimal TES option can then be identified based on its respective TAC ranking. A case study that compares the effectiveness of the indirect method against the intraplant and direct methods is conducted. The results show that among the 33 TES options evaluated, silica fire brick offers the lowest TAC and energy-related carbon emissions, leading to a reduction of 21.60% and 13.16%, respectively, as compared to the intraplant method. Subsequently, a sensitivity analysis is performed to explore the impacts of varying stream flowrates and storage capacity redundancy allocation on the TES selection. This provides insights into the performance of various TES options under intraplant, direct, and indirect heat integration methods. Finally, the threshold (i.e., stream flowrate required to provide economic gain under a given redundant allocation scenario) aligned with the strategic planning can be determined. This work demonstrates that TES integration can improve the economic feasibility and sustainability of industrial symbiosis in energy-intensive industries.

Open Access: Yes

DOI: 10.1016/j.tsep.2026.104707